234266

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3663999..3664693 Replicon chromosome
Accession NZ_CP091652
Organism Escherichia coli strain KMi025

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag L6L63_RS17770 Protein ID WP_001263489.1
Coordinates 3664295..3664693 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag L6L63_RS17765 Protein ID WP_000554758.1
Coordinates 3663999..3664292 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L63_RS17745 (3659631) 3659631..3660128 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
L6L63_RS17750 (3660352) 3660352..3662064 - 1713 Protein_3463 flagellar biosynthesis protein FlhA -
L6L63_RS17755 (3662036) 3662036..3662821 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
L6L63_RS17760 (3662892) 3662892..3663947 + 1056 WP_001226164.1 DNA polymerase IV -
L6L63_RS17765 (3663999) 3663999..3664292 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
L6L63_RS17770 (3664295) 3664295..3664693 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
L6L63_RS17775 (3664703) 3664703..3665155 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
L6L63_RS17780 (3665473) 3665473..3665679 + 207 Protein_3469 RtcB family protein -
L6L63_RS17785 (3665675) 3665675..3666196 + 522 Protein_3470 peptide chain release factor H -
L6L63_RS17790 (3666253) 3666253..3667710 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
L6L63_RS17795 (3667971) 3667971..3668429 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (3669025) 3669025..3669105 + 81 NuclAT_13 - -
- (3669025) 3669025..3669105 + 81 NuclAT_13 - -
- (3669025) 3669025..3669105 + 81 NuclAT_13 - -
- (3669025) 3669025..3669105 + 81 NuclAT_13 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T234266 WP_001263489.1 NZ_CP091652:3664295-3664693 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T234266 NZ_LR129843:c1119779-1119525 [Streptococcus pneumoniae]
TTGTATAAATTAGTTCCAACAAGACGTTTCATCAAGCAATTGAAAAAATTGGACCGTTATACGCAGAAGCTAATTACAAA
CTATTTACAAATCAATGTTTTGGAAGACCCAAGACGACACGGAAAGGCTTTGGTTGGTAATCGCGTTGGTCAATGGCGCT
ATAGAATTGGTAATTATCGAGTTATCGTACAAATTGTAGATGATGAATTAGTTGTTGCTACTCTAGAAGTTGGTCATCGG
AGAGATATTTATTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT234266 WP_000554758.1 NZ_CP091652:3663999-3664292 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT234266 NZ_LR129843:c1120023-1119781 [Streptococcus pneumoniae]
ATGACTACTATTACATTAAAAGTTTCTGAAGCTGATAAAACATTTATGAAAGCAATGGCTAAGTTTGAAGGAGTTTCCCT
TTCGGAACTTATTCGCACCAAAACTCTTGAAGCCCTAGAAGATGAATACGATGCTCGTGTGGCAGATTTAGCCTATCAAG
AGTATTTAGAAGACTTGGAAAAAGGAGTTGAACCCATTACTTGGGAAGAAATGATGCATGATTTAGGCTTGAAGGATGAA
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References