Detailed information of TA system TA234260

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 1871873..1872566
• Replicon: chromosome
• Accession: NZ_CP091652
• Organism: Escherichia coli strain KMi025

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: L6L63_RS09145
• Protein ID: WP_000415584.1
• Coordinates: 1872270..1872566 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: L6L63_RS09140
• Protein ID: WP_000650107.1
• Coordinates: 1871873..1872268 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L63_RS09130 (1867737)	1867737..1869995	-	2259	WP_049253102.1	DNA topoisomerase IV subunit A	-
L6L63_RS09135 (1870133)	1870133..1871740	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
L6L63_RS09140 (1871873)	1871873..1872268	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
L6L63_RS09145 (1872270)	1872270..1872566	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
L6L63_RS09150 (1872771)	1872771..1873253	-	483	WP_053272136.1	GyrI-like domain-containing protein	-
L6L63_RS09155 (1873306)	1873306..1873698	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
L6L63_RS09160 (1873850)	1873850..1874509	+	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
L6L63_RS09165 (1874506)	1874506..1875855	+	1350	WP_001618857.1	quorum sensing histidine kinase QseC	-
L6L63_RS09170 (1875901)	1875901..1876233	-	333	WP_000917684.1	DUF2645 family protein	-
L6L63_RS09175 (1876552)	1876552..1877133	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
L6L63_RS09180 (1877164)	1877164..1877478	+	315	WP_000958598.1	putative quinol monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1798045..1885881	87836

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T234260 WP_000415584.1 NZ_CP091652:c1872566-1872270 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T234260 NZ_LR129840:c1707903-1707730 [Streptococcus pneumoniae]
ATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCATATTAA
AATCGAGAAGCAAGGGGAAAGACCTATCACAATCCTTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAGGAAAGC
AAGCTGGGTTGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT234260 WP_000650107.1 NZ_CP091652:c1872268-1871873 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT234260 NZ_LR129840:c1707693-1707241 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACCATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65