Detailed information of TA system TA234256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 764141..764643
• Replicon: chromosome
• Accession: NZ_CP091652
• Organism: Escherichia coli strain KMi025

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A8E0FJZ4
• Locus tag: L6L63_RS03970
• Protein ID: WP_000767833.1
• Coordinates: 764141..764395 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: L6L63_RS03975
• Protein ID: WP_001259255.1
• Coordinates: 764392..764643 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L63_RS03940 (759156)	759156..759383	-	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-
L6L63_RS03945 (759397)	759397..760455	-	1059	WP_000492324.1	thiosulfate utilization transporter TsuA/YeeE	-
L6L63_RS03950 (760634)	760634..761992	-	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
L6L63_RS03955 (761982)	761982..762044	-	63	WP_010723108.1	membrane protein YoeI	-
L6L63_RS03960 (762259)	762259..763188	-	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
L6L63_RS03965 (763234)	763234..764058	-	825	WP_000754758.1	SDR family oxidoreductase	-
L6L63_RS03970 (764141)	764141..764395	-	255	WP_000767833.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
L6L63_RS03975 (764392)	764392..764643	-	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
L6L63_RS03980 (764926)	764926..764976	+	51	WP_001364200.1	his operon leader peptide	-
L6L63_RS03985 (765122)	765122..766021	+	900	WP_000131756.1	ATP phosphoribosyltransferase	-
L6L63_RS03990 (766027)	766027..767325	+	1299	WP_049290931.1	histidinol dehydrogenase	-
L6L63_RS03995 (767328)	767328..768398	+	1071	WP_000108940.1	histidinol-phosphate transaminase	-
L6L63_RS04000 (768398)	768398..769465	+	1068	WP_000080093.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10173.54 Da
• Isoelectric Point: 8.0353

>T234256 WP_000767833.1 NZ_CP091652:c764395-764141 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPESLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T234256 NZ_LR031521:1644934-1645269 [Streptococcus pyogenes]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGCTAAAAGAAATTCGTGATTATATTTCTCA
AAACTATTCCTCAACATCAGGTCAGCGTAAAATGGAGCAAATCATTAGCGACATAGAAAAACTCGAGGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAAAAATCATCCATTATCACAGCACTAAAGGTTATACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAGGGGGAAGAAAATAGGATCGTTATTGATTACTTGCTTCCTACTCAAAGCGACTATAT
AAAGTTATTCAAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT234256 WP_001259255.1 NZ_CP091652:c764643-764392 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT234256 NZ_LR031521:1644657-1644944 [Streptococcus pyogenes]
ATGACTACAGTAAAAAAAAACAGAGCGGTTACCTTTCAAGCTAATAAAGAATTGGTAAGCGAAGCAATGACAGTATTAAA
CAAAAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTACAAAATGTCGTTGTCACAAACGAGGTTGACTTATTGA
CGGAAGAGGAGTTAGAGAAAGAAAAACTTTTCAAGCAATTTCAAGCTGAAATCAACAAAAATATTGAGGATGTTCGTCAA
GGGAAGTTTTATACCTCTGAGGAAGTGAGGGCTGAACTTGGACTATAA

Structures

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0