Detailed information of TA system TA234251

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4968408..4969010
• Replicon: chromosome
• Accession: NZ_CP091651
• Organism: Escherichia coli strain KTa008

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: L6L70_RS23925
• Protein ID: WP_000897305.1
• Coordinates: 4968699..4969010 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L6L70_RS23920
• Protein ID: WP_000356395.1
• Coordinates: 4968408..4968698 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L70_RS23885 (4964032)	4964032..4964934	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
L6L70_RS23890 (4964931)	4964931..4965566	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
L6L70_RS23895 (4965563)	4965563..4966492	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
L6L70_RS23900 (4966674)	4966674..4966916	-	243	WP_001309881.1	CopG family transcriptional regulator	-
L6L70_RS23905 (4967135)	4967135..4967353	-	219	WP_001295676.1	CopG family transcriptional regulator	-
L6L70_RS23910 (4967772)	4967772..4968050	-	279	WP_001315112.1	hypothetical protein	-
L6L70_RS23915 (4968102)	4968102..4968323	-	222	WP_001550354.1	hypothetical protein	-
L6L70_RS23920 (4968408)	4968408..4968698	-	291	WP_000356395.1	NadS family protein	Antitoxin
L6L70_RS23925 (4968699)	4968699..4969010	-	312	WP_000897305.1	hypothetical protein	Toxin
L6L70_RS23930 (4969239)	4969239..4970147	+	909	WP_001550353.1	alpha/beta hydrolase	-
L6L70_RS23935 (4970315)	4970315..4971229	-	915	WP_109553727.1	transposase	-
L6L70_RS23940 (4971242)	4971242..4972129	-	888	Protein_4692	hypothetical protein	-
L6L70_RS23945 (4972545)	4972545..4973486	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
L6L70_RS23950 (4973531)	4973531..4973968	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T234251 WP_000897305.1 NZ_CP091651:c4969010-4968699 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T234251 NZ_LR027878:c2366893-2366684 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT234251 WP_000356395.1 NZ_CP091651:c4968698-4968408 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT234251 NZ_LR027878:c2367198-2366905 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7