Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4046698..4047392 Replicon chromosome
Accession NZ_CP091651
Organism Escherichia coli strain KTa008

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag L6L70_RS19590 Protein ID WP_001263491.1
Coordinates 4046698..4047096 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag L6L70_RS19595 Protein ID WP_000554755.1
Coordinates 4047099..4047392 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (4042527) 4042527..4042607 - 81 NuclAT_11 - -
- (4042527) 4042527..4042607 - 81 NuclAT_11 - -
- (4042527) 4042527..4042607 - 81 NuclAT_11 - -
- (4042527) 4042527..4042607 - 81 NuclAT_11 - -
L6L70_RS19560 (4041867) 4041867..4043111 - 1245 WP_000189541.1 esterase FrsA -
L6L70_RS19565 (4043203) 4043203..4043661 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
L6L70_RS19570 (4043922) 4043922..4045379 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
L6L70_RS19575 (4045436) 4045436..4045788 - 353 Protein_3846 peptide chain release factor H -
L6L70_RS19580 (4045784) 4045784..4045990 - 207 Protein_3847 RtcB family protein -
L6L70_RS19585 (4046236) 4046236..4046688 - 453 WP_019842500.1 GNAT family N-acetyltransferase -
L6L70_RS19590 (4046698) 4046698..4047096 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
L6L70_RS19595 (4047099) 4047099..4047392 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
L6L70_RS19600 (4047444) 4047444..4048499 - 1056 WP_001550655.1 DNA polymerase IV -
L6L70_RS19605 (4048570) 4048570..4049355 - 786 WP_019842510.1 putative lateral flagellar export/assembly protein LafU -
L6L70_RS19610 (4049327) 4049327..4051039 + 1713 Protein_3853 flagellar biosynthesis protein FlhA -
L6L70_RS19615 (4051144) 4051144..4051422 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
L6L70_RS19620 (4051415) 4051415..4051771 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4036634..4047392 10758


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T234247 WP_001263491.1 NZ_CP091651:c4047096-4046698 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T234247 NZ_LR027878:c2017582-2017430 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT234247 WP_000554755.1 NZ_CP091651:c4047392-4047099 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT234247 NZ_LR027878:c2018206-2017607 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References