Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1235723..1236450 Replicon chromosome
Accession NZ_CP091651
Organism Escherichia coli strain KTa008

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag L6L70_RS06000 Protein ID WP_000547555.1
Coordinates 1235723..1236034 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag L6L70_RS06005 Protein ID WP_000126294.1
Coordinates 1236031..1236450 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L70_RS05975 (1231658) 1231658..1233367 + 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -
L6L70_RS05980 (1233377) 1233377..1233919 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
L6L70_RS05985 (1233919) 1233919..1234686 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
L6L70_RS05990 (1234683) 1234683..1235093 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
L6L70_RS05995 (1235086) 1235086..1235556 + 471 WP_001551542.1 hydrogenase maturation peptidase HycI -
L6L70_RS06000 (1235723) 1235723..1236034 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
L6L70_RS06005 (1236031) 1236031..1236450 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
L6L70_RS06010 (1236529) 1236529..1237953 - 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
L6L70_RS06015 (1237962) 1237962..1239419 - 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
L6L70_RS06020 (1239679) 1239679..1240689 + 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
L6L70_RS06025 (1240838) 1240838..1241365 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T234230 WP_000547555.1 NZ_CP091651:1235723-1236034 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T234230 NZ_LR027876:c2595239-2595132 [Staphylococcus aureus]
ATGTTCAATTTATTAATTAACATCATGACTTCAGCTATAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT234230 WP_000126294.1 NZ_CP091651:1236031-1236450 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT234230 NZ_LR027876:2595055-2595115 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References