Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 3130278..3130864 Replicon chromosome
Accession NZ_CP091650
Organism Klebsiella pneumoniae strain TAKPN-1

Toxin (Protein)


Gene name doc Uniprot ID A0A331ME34
Locus tag L6D56_RS15035 Protein ID WP_025861226.1
Coordinates 3130496..3130864 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag L6D56_RS15030 Protein ID WP_004174006.1
Coordinates 3130278..3130499 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6D56_RS15010 3126434..3127360 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
L6D56_RS15015 3127357..3128634 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
L6D56_RS15020 3128631..3129398 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
L6D56_RS15025 3129400..3130113 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
L6D56_RS15030 3130278..3130499 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
L6D56_RS15035 3130496..3130864 + 369 WP_025861226.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
L6D56_RS15040 3131137..3132453 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
L6D56_RS15045 3132560..3133447 + 888 WP_014906831.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
L6D56_RS15050 3133444..3134289 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
L6D56_RS15055 3134291..3135361 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3127357..3136098 8741


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-121)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13551.98 Da        Isoelectric Point: 8.6410

>T234213 WP_025861226.1 NZ_CP091650:3130496-3130864 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGIKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T234213 NZ_LR027874:c2072686-2072510 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTCATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT234213 WP_004174006.1 NZ_CP091650:3130278-3130499 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT234213 NZ_LR027874:2072387-2072442 [Staphylococcus aureus]
AAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331ME34

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References