Detailed information of TA system TA234171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4452637..4453391
• Replicon: chromosome
• Accession: NZ_CP091636
• Organism: Salmonella enterica strain 1063

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: L5504_RS21250
• Protein ID: WP_133323219.1
• Coordinates: 4452637..4452948 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L5504_RS21255
• Protein ID: WP_001259009.1
• Coordinates: 4452945..4453391 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5504_RS21220 (4448295)	4448295..4449197	+	903	WP_000331364.1	formate dehydrogenase subunit beta	-
L5504_RS21225 (4449194)	4449194..4449829	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
L5504_RS21230 (4449826)	4449826..4450755	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
L5504_RS21235 (4450802)	4450802..4451092	-	291	WP_000362050.1	DNA-binding transcriptional regulator	-
L5504_RS21240 (4451093)	4451093..4451404	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
L5504_RS21245 (4451622)	4451622..4452551	+	930	WP_001127694.1	alpha/beta hydrolase	-
L5504_RS21250 (4452637)	4452637..4452948	+	312	WP_133323219.1	type II toxin-antitoxin system HigB family toxin	Toxin
L5504_RS21255 (4452945)	4452945..4453391	+	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
L5504_RS21260 (4453406)	4453406..4454347	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
L5504_RS21265 (4454392)	4454392..4454829	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
L5504_RS21270 (4454826)	4454826..4455698	-	873	WP_000921423.1	virulence factor BrkB family protein	-
L5504_RS21275 (4455692)	4455692..4456291	-	600	WP_000965698.1	glucose-1-phosphatase	-
L5504_RS21280 (4456482)	4456482..4457285	-	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
L5504_RS21285 (4457319)	4457319..4458215	-	897	WP_001520529.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12490.44 Da
• Isoelectric Point: 9.1995

>T234171 WP_133323219.1 NZ_CP091636:4452637-4452948 [Salmonella enterica]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSDNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T234171 NZ_LR027557:c1919953-1919612 [Pseudomonas synxantha]
ATGACAACACTGACAATCCGCTACACCGAAATTGCACAACAGAGCATCGAAGACCAGGTCGAGCATCTGGCCGAGTATCA
CAGCCTCGACTACGCCCTCCATCGGCTAGACACTGTGATCGACGTCCTTCAGAAAAAGCTTTTATCCACCCCGTTGGGCT
ACCCAATCAGCTCTCAAGCCAGCGAGCTGGGCATCCTGCATTACCGTGAACTCAACACCGATGGATACCGGGTGTTCTAC
GAGATCGTGGAGGCGGACCAGGCGATTGTCGTTGCACTGGTGCTGCGGGATAAACAAAGCGTCGAAAAGGCACTGGTTCG
TCACTGTCTACTGCATACGTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16720.06 Da
• Isoelectric Point: 6.6451

>AT234171 WP_001259009.1 NZ_CP091636:4452945-4453391 [Salmonella enterica]
MRTHRQMDATSAKKIVDTFSDAVKSVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT234171 NZ_LR027557:c1920215-1919967 [Pseudomonas synxantha]
ATGCGAGTTGAAACAGTTAGCTTTTTAAAAAAATACGCATCCGACCTGCCTCTAGATGAGCCAATGGTCGTCACGCAAAA
CGGTGTACCAGCATACGTTGTGGAGTCTTATGCCGAGCATAAGCGCCGCGATGAAACGGTAGCCCTGGTGAAATTACTGG
CCATGAGCTCTCGCCAGTATGCCCAAGGCAAGCATTGTTCGACTGATGAGCTGAAAGCCCGCATGAGCAGGAGGTTTGCC
CACAAATAA