Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 460699..461210 Replicon chromosome
Accession NC_011750
Organism Escherichia coli IAI39

Toxin (Protein)


Gene name yafQ Uniprot ID A0A0H3MIA4
Locus tag ECIAI39_RS02115 Protein ID WP_012602203.1
Coordinates 460962..461210 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q32JN2
Locus tag ECIAI39_RS02110 Protein ID WP_000729709.1
Coordinates 460699..460959 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECIAI39_RS02090 456486..457271 - 786 WP_000207579.1 putative lateral flagellar export/assembly protein LafU -
ECIAI39_RS02095 457243..458950 + 1708 Protein_417 flagellar biosynthesis protein FlhA -
ECIAI39_RS02100 459067..459564 - 498 WP_000006244.1 transposase -
ECIAI39_RS02105 459740..460513 - 774 WP_015674584.1 C40 family peptidase -
ECIAI39_RS02110 460699..460959 + 261 WP_000729709.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
ECIAI39_RS02115 460962..461210 + 249 WP_012602203.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
ECIAI39_RS02125 461272..462428 + 1157 WP_096974168.1 IS3-like element IS600 family transposase -
ECIAI39_RS02130 462663..463403 + 741 WP_001225679.1 murein L,D-transpeptidase -
ECIAI39_RS02135 463374..464141 - 768 WP_000333380.1 class II glutamine amidotransferase -
ECIAI39_RS02140 464246..464824 - 579 WP_001570958.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 459740..472027 12287


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-81)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9834.53 Da        Isoelectric Point: 9.8885

>T23416 WP_012602203.1 NC_011750:460962-461210 [Escherichia coli IAI39]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLLLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FE

Download         Length: 249 bp

>T23416 NC_011750:460962-461210 [Escherichia coli IAI39]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACTGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9333.73 Da        Isoelectric Point: 5.8129

>AT23416 WP_000729709.1 NC_011750:460699-460959 [Escherichia coli IAI39]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVGVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT23416 NC_011750:460699-460959 [Escherichia coli IAI39]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACTATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3MIA4

Antitoxin

Source ID Structure
AlphaFold DB A0A829FY17

References