Detailed information of TA system TA023408

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4460067..4460669
• Replicon: chromosome
• Accession: NC_011748
• Organism: Escherichia coli 55989

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: EC55989_RS22275
• Protein ID: WP_000897305.1
• Coordinates: 4460067..4460378 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EC55989_RS22280
• Protein ID: WP_000356397.1
• Coordinates: 4460379..4460669 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC55989_RS22250	4455981..4456580	+	600	WP_001295269.1	glucose-1-phosphatase	-
EC55989_RS22255	4456574..4457446	+	873	WP_000920762.1	virulence factor BrkB family protein	-
EC55989_RS22260	4457443..4457880	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EC55989_RS22265	4457925..4458866	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
EC55989_RS22270	4458930..4459838	-	909	WP_001162704.1	alpha/beta hydrolase	-
EC55989_RS22275	4460067..4460378	+	312	WP_000897305.1	hypothetical protein	Toxin
EC55989_RS22280	4460379..4460669	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
EC55989_RS22285	4461255..4461473	+	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
EC55989_RS22290	4461693..4461935	+	243	WP_001087409.1	hypothetical protein	-
EC55989_RS22300	4462265..4463194	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
EC55989_RS22305	4463191..4463826	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
EC55989_RS22310	4463823..4464725	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T23408 WP_000897305.1 NC_011748:4460067-4460378 [Escherichia coli 55989]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T23408 NC_011748:4460067-4460378 [Escherichia coli 55989]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT23408 WP_000356397.1 NC_011748:4460379-4460669 [Escherichia coli 55989]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT23408 NC_011748:4460379-4460669 [Escherichia coli 55989]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTCAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7