Detailed information of TA system TA023407

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3631969..3632768
• Replicon: chromosome
• Accession: NC_011748
• Organism: Escherichia coli 55989

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: U9XVR9
• Locus tag: EC55989_RS18180
• Protein ID: WP_000347267.1
• Coordinates: 3632304..3632768 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: EC55989_RS18175
• Protein ID: WP_001307405.1
• Coordinates: 3631969..3632304 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC55989_RS18155	3627755..3628525	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
EC55989_RS18160	3628541..3629875	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
EC55989_RS18170	3630249..3631820	+	1572	WP_001273741.1	galactarate dehydratase	-
EC55989_RS18175	3631969..3632304	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
EC55989_RS18180	3632304..3632768	+	465	WP_000347267.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
EC55989_RS18185	3632823..3633632	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
EC55989_RS18190	3633881..3635161	+	1281	WP_000681935.1	tagatose-bisphosphate aldolase subunit KbaZ	-
EC55989_RS18195	3635184..3635657	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
EC55989_RS18200	3635668..3636447	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
EC55989_RS18205	3636437..3637315	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
EC55989_RS18210	3637333..3637767	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3619703..3632768	13065

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17807.17 Da
• Isoelectric Point: 9.4947

>T23407 WP_000347267.1 NC_011748:3632304-3632768 [Escherichia coli 55989]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T23407 NC_011748:3632304-3632768 [Escherichia coli 55989]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT23407 WP_001307405.1 NC_011748:3631969-3632304 [Escherichia coli 55989]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT23407 NC_011748:3631969-3632304 [Escherichia coli 55989]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0XTR4

Antitoxin

• AlphaFold DB: A0A0E0XVC7