Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1866970..1867567 Replicon chromosome
Accession NZ_CP091601
Organism Klebsiella pneumoniae strain Nord77_R886

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag L5469_RS10340 Protein ID WP_004142563.1
Coordinates 1866970..1867287 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag L5469_RS10345 Protein ID WP_004142561.1
Coordinates 1867280..1867567 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5469_RS10325 (1862673) 1862673..1864100 - 1428 WP_004176980.1 MFS transporter -
L5469_RS10330 (1864209) 1864209..1865075 + 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
L5469_RS10335 (1865742) 1865742..1866785 + 1044 WP_004178895.1 DUF2157 domain-containing protein -
L5469_RS10340 (1866970) 1866970..1867287 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L5469_RS10345 (1867280) 1867280..1867567 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
L5469_RS10350 (1867835) 1867835..1868485 - 651 Protein_1710 oxygen-insensitive NAD(P)H nitroreductase -
L5469_RS10355 (1868605) 1868605..1868973 - 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
L5469_RS10360 (1868973) 1868973..1869554 - 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
L5469_RS10365 (1869734) 1869734..1870849 + 1116 WP_020316605.1 MBL fold metallo-hydrolase -
L5469_RS10370 (1870880) 1870880..1871221 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
L5469_RS10375 (1871239) 1871239..1871487 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T233944 WP_004142563.1 NZ_CP091601:1866970-1867287 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T233944 NZ_LN879429:c810447-810175 [Bartonella henselae]
ATGAAGCTCGTTTGGACACGGATAGCGCATTACGATCGTCAAAAGATACGGGAATATATCGCGCAAGAAAATCCTTCTGC
TGCATTAGTATTCGACAAGCTTTTATCTAAAAAAGTAGAAAGACTCATTAGCTTTCCTAATCTTGGTCGTGTTGGCCGAA
TTAGGAATACGCGCGAGCTTATTGTGCGTAAGAATTATATTATGATTTATGATGTTTCTAATGGCACTATACGGGTTTTG
CGCATACTTCACGCAAAACAAAAGTGGCCATAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT233944 WP_004142561.1 NZ_CP091601:1867280-1867567 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT233944 NZ_LN879429:c810734-810444 [Bartonella henselae]
ATGGCTGAAACAACATTTACCTTTCGCGTTGATGATGCATTAAAAAGTGAATTTTCTCAGGCTGCAAAAGCATGTGATCG
ATCTGCTGCACAGCTCTTACGGGATTACATGCGTGATATTGTAAAAGAACAAAAAGAAAAGATAGCGCATGAATTATGGT
TTCAGGAACAAGTCCAACTTGGATTAAATTCTGCCAACGCAGGTGATGTAATACCTTTTGAAGAAATTGAAACAGAAGCA
CAAGCATGGCGCTTTGAAATACAACGCAAGCTGAAGACATCAGATTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References