Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1429870..1430516 Replicon chromosome
Accession NZ_CP091594
Organism Klebsiella pneumoniae strain Nord5-1_R48

Toxin (Protein)


Gene name higB Uniprot ID A0A4S7KZ03
Locus tag L5467_RS09835 Protein ID WP_032433636.1
Coordinates 1429870..1430217 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4S7L580
Locus tag L5467_RS09840 Protein ID WP_019725405.1
Coordinates 1430217..1430516 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5467_RS09825 (1425796) 1425796..1427229 + 1434 WP_032433640.1 glycogen synthase GlgA -
L5467_RS09830 (1427247) 1427247..1429694 + 2448 WP_032433638.1 glycogen phosphorylase -
L5467_RS09835 (1429870) 1429870..1430217 + 348 WP_032433636.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L5467_RS09840 (1430217) 1430217..1430516 + 300 WP_019725405.1 XRE family transcriptional regulator Antitoxin
L5467_RS09845 (1430579) 1430579..1432087 - 1509 WP_029602548.1 glycerol-3-phosphate dehydrogenase -
L5467_RS09850 (1432292) 1432292..1432621 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
L5467_RS09855 (1432672) 1432672..1433502 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
L5467_RS09860 (1433552) 1433552..1434310 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13500.51 Da        Isoelectric Point: 6.2327

>T233921 WP_032433636.1 NZ_CP091594:1429870-1430217 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGINEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T233921 NZ_LN865164:c2779908-2779600 [Pseudomonas sp. URMO17WK12:I11]
ATGTGCATAAGCACCGTCACCTTGATGGAGTTGATCTATGGTGCTGAGAAATCCTCGAATTCCGAGCGTAATCTAACAGC
GGTTGAAGGCCTGGCAGCTCGCTTGGAAGTTCTTACATATGATAGAAACGCTGCCGCCCACACTGGACAGCTCCGTGTTG
AATTGGCACGTTTGGGCACGCCTATTGGCCCTTTCGATCAAATGATCGCTGGTCACGCTCGCTCACAAGGTCTGATTATT
GTCACTAATAACCGTCGTGAGTTTGAGCGAGTACCTGGGCTACGGAGCGAAGACTGGGTTATCCGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10775.53 Da        Isoelectric Point: 9.7681

>AT233921 WP_019725405.1 NZ_CP091594:1430217-1430516 [Klebsiella pneumoniae]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNDPRLSTLKSYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT233921 NZ_LN865164:c2780169-2779939 [Pseudomonas sp. URMO17WK12:I11]
ATGGAACAAGGTGCCGTTTTTTTGAGTAATCGTAGCCAAGCTATAAGACTGCCCAAAGCTGCAGCATTTTCGGAAGACGT
CAAGCGCGTTGACGTCGTCGCGATTGGGCGCATACGCATCATCACACCTGCCGGGGAAGCATGGGATAGCTGGTTCGACG
ACTTAGCCCCTACAGAAGATTTCATGACAGAGCGTGATCAGTCGGCTGATCAAGAGCGCGAGCGTTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S7KZ03

Antitoxin

Source ID Structure
AlphaFold DB A0A4S7L580

References