Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 278667..279208 Replicon chromosome
Accession NC_011748
Organism Escherichia coli 55989

Toxin (Protein)


Gene name yafQ Uniprot ID B7LHF6
Locus tag EC55989_RS01275 Protein ID WP_000615974.1
Coordinates 278667..278945 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag EC55989_RS01280 Protein ID WP_000729704.1
Coordinates 278948..279208 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC55989_RS01260 276249..276827 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
EC55989_RS01265 277033..277800 + 768 WP_000333380.1 class II glutamine amidotransferase -
EC55989_RS01270 277771..278511 - 741 WP_001225679.1 murein L,D-transpeptidase -
EC55989_RS01275 278667..278945 - 279 WP_000615974.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EC55989_RS01280 278948..279208 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EC55989_RS01285 279394..280167 + 774 WP_000543895.1 C40 family peptidase -
EC55989_RS01290 280224..280580 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
EC55989_RS01295 280573..280851 - 279 WP_000598758.1 type II toxin-antitoxin system HicA family toxin -
EC55989_RS01300 280956..282668 - 1713 Protein_246 flagellar biosynthesis protein FlhA -
EC55989_RS01305 282640..283425 + 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10855.68 Da        Isoelectric Point: 10.2267

>T23390 WP_000615974.1 NC_011748:c278945-278667 [Escherichia coli 55989]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMRLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T23390 NC_011748:c278945-278667 [Escherichia coli 55989]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGAGGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT23390 WP_000729704.1 NC_011748:c279208-278948 [Escherichia coli 55989]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT23390 NC_011748:c279208-278948 [Escherichia coli 55989]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y514

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References