Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2195031..2195677 Replicon chromosome
Accession NZ_CP091582
Organism Klebsiella pneumoniae strain Nord9_R85

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag L5468_RS10625 Protein ID WP_002920560.1
Coordinates 2195031..2195378 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag L5468_RS10630 Protein ID WP_002920557.1
Coordinates 2195378..2195677 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5468_RS10615 (L5468_10615) 2190957..2192390 + 1434 WP_002920564.1 glycogen synthase GlgA -
L5468_RS10620 (L5468_10620) 2192408..2194855 + 2448 WP_002920561.1 glycogen phosphorylase -
L5468_RS10625 (L5468_10625) 2195031..2195378 + 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L5468_RS10630 (L5468_10630) 2195378..2195677 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
L5468_RS10635 (L5468_10635) 2195740..2197248 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
L5468_RS10640 (L5468_10640) 2197453..2197782 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
L5468_RS10645 (L5468_10645) 2197833..2198663 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
L5468_RS10650 (L5468_10650) 2198713..2199471 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T233899 WP_002920560.1 NZ_CP091582:2195031-2195378 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T233899 NZ_LN854556:c823139-822951 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT233899 WP_002920557.1 NZ_CP091582:2195378-2195677 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT233899 NZ_LN854556:c823428-823168 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACAACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATTACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCGATT
TTTTATAGGTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References