Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 2168844..2169430 Replicon chromosome
Accession NZ_CP091582
Organism Klebsiella pneumoniae strain Nord9_R85

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag L5468_RS10515 Protein ID WP_002920800.1
Coordinates 2169062..2169430 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A0H3GZM4
Locus tag L5468_RS10510 Protein ID WP_002920802.1
Coordinates 2168844..2169065 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5468_RS10490 (L5468_10490) 2165001..2165927 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
L5468_RS10495 (L5468_10495) 2165924..2167201 + 1278 WP_002920806.1 branched chain amino acid ABC transporter permease LivM -
L5468_RS10500 (L5468_10500) 2167198..2167965 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
L5468_RS10505 (L5468_10505) 2167967..2168680 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
L5468_RS10510 (L5468_10510) 2168844..2169065 + 222 WP_002920802.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
L5468_RS10515 (L5468_10515) 2169062..2169430 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
L5468_RS10520 (L5468_10520) 2169703..2171019 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
L5468_RS10525 (L5468_10525) 2171126..2172013 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
L5468_RS10530 (L5468_10530) 2172010..2172855 + 846 WP_002920789.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
L5468_RS10535 (L5468_10535) 2172857..2173927 + 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2167198..2174664 7466


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-49)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T233898 WP_002920800.1 NZ_CP091582:2169062-2169430 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T233898 NZ_LN854556:652616-652792 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGTTGGCATTACTGAAATCTTTAGAAAGGAGATGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGTTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8181.18 Da        Isoelectric Point: 4.7769

>AT233898 WP_002920802.1 NZ_CP091582:2168844-2169065 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT233898 NZ_LN854556:c652954-652780 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZM4

References