Detailed information of TA system TA023385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 421959..422592
• Replicon: chromosome
• Accession: NC_011744
• Organism: Vibrio splendidus LGP32

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A347UQS4
• Locus tag: VS_RS16600
• Protein ID: WP_012600340.1
• Coordinates: 421959..422291 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: VS_RS16605
• Protein ID: WP_012600341.1
• Coordinates: 422278..422592 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VS_RS16570	417691..418044	+	354	WP_009845655.1	hypothetical protein	-
VS_RS16575	418141..418812	+	672	WP_041473056.1	DUF3313 domain-containing protein	-
VS_RS16580	418825..419043	+	219	WP_009845653.1	DUF3820 family protein	-
VS_RS16585	419627..420304	+	678	WP_012600337.1	hypothetical protein	-
VS_RS16590	420668..420937	-	270	Protein_355	IS110 family transposase	-
VS_RS16595	421035..421463	+	429	WP_012600339.1	hypothetical protein	-
VS_RS16600	421959..422291	+	333	WP_012600340.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VS_RS16605	422278..422592	+	315	WP_012600341.1	DNA-binding transcriptional regulator	Antitoxin
VS_RS16610	423182..423523	+	342	WP_080518029.1	hypothetical protein	-
VS_RS16615	423507..423962	+	456	WP_041473058.1	hypothetical protein	-
VS_RS22350	424300..424497	-	198	Protein_361	IS110 family transposase	-
VS_RS16620	424597..425118	+	522	WP_012600345.1	hypothetical protein	-
VS_RS16625	425805..426857	+	1053	WP_012600346.1	3-deoxy-7-phosphoheptulonate synthase AroG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	420608..420937	329

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-104)

Antitoxin

• Antitoxin MqsA/HigA-2 (18-101)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12906.76 Da
• Isoelectric Point: 10.0960

>T23385 WP_012600340.1 NC_011744:421959-422291 [Vibrio atlanticus]
MKSVFVESTIFEKYRNEYLSDDEFRLFQAELMSNPKQGDVIQGTGGLRKIRVASKGKGKRGGSRVIYYFLDEKRRFYLLT
IYGKNEMSDLTADQKKQLKAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T23385 NC_011744:421959-422291 [Vibrio atlanticus]
ATGAAAAGTGTATTTGTCGAATCGACCATATTTGAAAAGTACCGTAATGAATATCTCAGCGATGATGAGTTTAGGCTTTT
TCAAGCTGAATTAATGTCTAACCCAAAACAGGGGGACGTGATTCAAGGTACAGGTGGTTTGCGAAAGATTCGAGTTGCAA
GTAAAGGTAAGGGCAAGCGAGGCGGCTCTCGTGTTATCTATTACTTCCTCGATGAAAAGCGTCGTTTCTACTTGCTGACA
ATTTATGGCAAGAATGAAATGTCCGATTTAACTGCAGATCAAAAGAAACAATTAAAGGCTTTTATGGAGGCGTGGCGTAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11747.24 Da
• Isoelectric Point: 6.6448

>AT23385 WP_012600341.1 NC_011744:422278-422592 [Vibrio atlanticus]
MSNRDLFAELSTALVEAKEHSESKLTLKTHQVNNISELDISPNEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSAP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT23385 NC_011744:422278-422592 [Vibrio atlanticus]
ATGAGCAATCGTGACCTATTTGCAGAGTTAAGTACTGCTCTTGTTGAGGCTAAAGAGCATTCAGAGAGTAAACTTACTCT
TAAAACTCATCAAGTTAACAATATCAGTGAGTTAGATATTTCACCGAATGAAATCGTGAGTATCCGTGAACAGTTTAATA
TGTCGCGTGGTGTGTTTGCGCGTTTACTACATACATCATCCCGAACATTAGAAAACTGGGAACAAGGGCGTAGCGCACCA
AATGGACAAGCTGTGACCCTTCTAAAATTGGTTCAGCGTCATCCGGAAACTCTTTCGCACATCGCTGAGCTATAA

Structures

Toxin

• AlphaFold DB: A0A347UQS4