Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4714321..4715075 Replicon chromosome
Accession NZ_CP091556
Organism Salmonella enterica strain 632

Toxin (Protein)


Gene name higB Uniprot ID B5F003
Locus tag INN80_RS23215 Protein ID WP_000558166.1
Coordinates 4714321..4714632 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag INN80_RS23220 Protein ID WP_001259011.1
Coordinates 4714629..4715075 (+) Length 149 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
INN80_RS23185 (4709979) 4709979..4710881 + 903 WP_000331361.1 formate dehydrogenase subunit beta -
INN80_RS23190 (4710878) 4710878..4711513 + 636 WP_000829025.1 formate dehydrogenase cytochrome b556 subunit -
INN80_RS23195 (4711510) 4711510..4712439 + 930 WP_000027730.1 formate dehydrogenase accessory protein FdhE -
INN80_RS23200 (4712486) 4712486..4712776 - 291 WP_000362050.1 DNA-binding transcriptional regulator -
INN80_RS23205 (4712777) 4712777..4713088 - 312 WP_001159630.1 cytotoxic translational repressor of toxin-antitoxin stability system -
INN80_RS23210 (4713306) 4713306..4714235 + 930 WP_001127703.1 alpha/beta hydrolase -
INN80_RS23215 (4714321) 4714321..4714632 + 312 WP_000558166.1 type II toxin-antitoxin system HigB family toxin Toxin
INN80_RS23220 (4714629) 4714629..4715075 + 447 WP_001259011.1 type II toxin-antitoxin system HigA family antitoxin Antitoxin
INN80_RS23225 (4715090) 4715090..4716031 - 942 WP_001518251.1 fatty acid biosynthesis protein FabY -
INN80_RS23230 (4716076) 4716076..4716513 - 438 WP_000560974.1 D-aminoacyl-tRNA deacylase -
INN80_RS23235 (4716510) 4716510..4717382 - 873 WP_249001048.1 virulence factor BrkB family protein -
INN80_RS23240 (4717376) 4717376..4717975 - 600 WP_000965695.1 glucose-1-phosphatase -
INN80_RS23245 (4718166) 4718166..4718969 - 804 WP_000059693.1 DeoR/GlpR family DNA-binding transcription regulator -
INN80_RS23250 (4719003) 4719003..4719899 - 897 WP_001520529.1 sugar kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(93-146)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12432.40 Da        Isoelectric Point: 9.5334

>T233721 WP_000558166.1 NZ_CP091556:4714321-4714632 [Salmonella enterica]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Download         Length: 312 bp

>T233721 NZ_LN614756:1650060-1650212 [Clostridioides difficile]
ATGGATAATTTTTTACAAGGTGTACTAGCAAGTTTAGTTGCCAGTTTAATAGTTTACTTAAATAGTAAATTGTTTAAAAA
AGTAAAAAGCCACTCTGGCAGGAGTGACTTTAGTTTTGAACTAAAAATCAAGTTCAAAAAGAATAAACATTAG

Antitoxin


Download         Length: 149 a.a.        Molecular weight: 16734.08 Da        Isoelectric Point: 6.6451

>AT233721 WP_001259011.1 NZ_CP091556:4714629-4715075 [Salmonella enterica]
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Download         Length: 447 bp

>AT233721 NZ_LN614756:c1650256-1650156 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTAGACGTAGAGTGAAGTTCAAATACTAATGTTTATTCTTTTTGAACTTGATTTTTAGTTC
AAAACTAAAGTCACTCCTGCC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R9ME90

Antitoxin

Source ID Structure

References