Detailed information of TA system TA233695

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4939350..4940104
• Replicon: chromosome
• Accession: NZ_CP091554
• Organism: Salmonella enterica strain 751

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5F003
• Locus tag: INN81_RS24165
• Protein ID: WP_000558166.1
• Coordinates: 4939793..4940104 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: INN81_RS24160
• Protein ID: WP_001259011.1
• Coordinates: 4939350..4939796 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INN81_RS24130 (4934526)	4934526..4935422	+	897	WP_001520529.1	sugar kinase	-
INN81_RS24135 (4935456)	4935456..4936259	+	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
INN81_RS24140 (4936450)	4936450..4937049	+	600	WP_000965695.1	glucose-1-phosphatase	-
INN81_RS24145 (4937043)	4937043..4937915	+	873	WP_000921427.1	virulence factor BrkB family protein	-
INN81_RS24150 (4937912)	4937912..4938349	+	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
INN81_RS24155 (4938394)	4938394..4939335	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
INN81_RS24160 (4939350)	4939350..4939796	-	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
INN81_RS24165 (4939793)	4939793..4940104	-	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	Toxin
INN81_RS24170 (4940190)	4940190..4941119	-	930	WP_001127703.1	alpha/beta hydrolase	-
INN81_RS24175 (4941337)	4941337..4941648	+	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
INN81_RS24180 (4941649)	4941649..4941939	+	291	WP_000362050.1	DNA-binding transcriptional regulator	-
INN81_RS24185 (4941986)	4941986..4942915	-	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
INN81_RS24190 (4942912)	4942912..4943547	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
INN81_RS24195 (4943544)	4943544..4944446	-	903	WP_000331361.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12432.40 Da
• Isoelectric Point: 9.5334

>T233695 WP_000558166.1 NZ_CP091554:c4940104-4939793 [Salmonella enterica]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T233695 NZ_LM995446:1458183-1458359 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16734.08 Da
• Isoelectric Point: 6.6451

>AT233695 WP_001259011.1 NZ_CP091554:c4939796-4939350 [Salmonella enterica]
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT233695 NZ_LM995446:1458405-1458821 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0R9ME90