Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2089245..2089747 Replicon chromosome
Accession NC_011742
Organism Escherichia coli S88

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ECS88_RS11945 Protein ID WP_000767829.1
Coordinates 2089245..2089499 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ECS88_RS11950 Protein ID WP_001259255.1
Coordinates 2089496..2089747 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECS88_RS11920 2084260..2084487 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
ECS88_RS11925 2084501..2085559 - 1059 WP_000492340.1 transport protein YeeE -
ECS88_RS11930 2085738..2087096 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ECS88_RS27360 2087086..2087148 - 63 WP_010723108.1 membrane protein YoeI -
ECS88_RS11935 2087363..2088292 - 930 WP_000803341.1 LysR family transcriptional regulator -
ECS88_RS11940 2088338..2089162 - 825 WP_000754755.1 SDR family oxidoreductase -
ECS88_RS11945 2089245..2089499 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ECS88_RS11950 2089496..2089747 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ECS88_RS11955 2090031..2090081 + 51 WP_001364200.1 his operon leader peptide -
ECS88_RS11965 2090227..2091126 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ECS88_RS11970 2091132..2092436 + 1305 WP_001514311.1 histidinol dehydrogenase -
ECS88_RS11975 2092433..2093503 + 1071 WP_000108976.1 histidinol-phosphate transaminase -
ECS88_RS11980 2093503..2094570 + 1068 WP_000080095.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T23368 WP_000767829.1 NC_011742:c2089499-2089245 [Escherichia coli S88]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T23368 NC_011742:c2089499-2089245 [Escherichia coli S88]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT23368 WP_001259255.1 NC_011742:c2089747-2089496 [Escherichia coli S88]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT23368 NC_011742:c2089747-2089496 [Escherichia coli S88]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References