Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1561003..1561565 Replicon chromosome
Accession NC_011742
Organism Escherichia coli S88

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag ECS88_RS09075 Protein ID WP_000605675.1
Coordinates 1561287..1561565 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag ECS88_RS09070 Protein ID WP_000781370.1
Coordinates 1561003..1561287 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECS88_RS09055 1556184..1559231 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
ECS88_RS09060 1559244..1560128 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
ECS88_RS09065 1560121..1560774 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
ECS88_RS09070 1561003..1561287 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
ECS88_RS09075 1561287..1561565 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ECS88_RS09080 1561751..1562761 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
ECS88_RS09085 1562895..1564592 - 1698 WP_000433462.1 malate dehydrogenase -
ECS88_RS09090 1564748..1564885 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
ECS88_RS09095 1564987..1565202 - 216 WP_000495766.1 biofilm-dependent modulation protein -
ECS88_RS09105 1565547..1565978 + 432 WP_000152310.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T23358 WP_000605675.1 NC_011742:c1561565-1561287 [Escherichia coli S88]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T23358 NC_011742:c1561565-1561287 [Escherichia coli S88]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT23358 WP_000781370.1 NC_011742:c1561287-1561003 [Escherichia coli S88]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT23358 NC_011742:c1561287-1561003 [Escherichia coli S88]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References