Detailed information of TA system TA233561

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 62305..62559
• Replicon: plasmid p537-20_1
• Accession: NZ_CP091535
• Organism: Escherichia coli strain 537-20

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: L6D13_RS24235
• Protein ID: WP_001312851.1
• Coordinates: 62305..62454 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	62498..62559 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6D13_RS24200 (57557)	57557..58672	+	1116	Protein_52	IS91 family transposase	-
L6D13_RS24205 (58853)	58853..59197	-	345	WP_001553768.1	hypothetical protein	-
L6D13_RS24210 (60133)	60133..60990	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
L6D13_RS24215 (60983)	60983..61465	-	483	WP_001273588.1	hypothetical protein	-
L6D13_RS24220 (61458)	61458..61505	-	48	WP_229471593.1	hypothetical protein	-
L6D13_RS24225 (61496)	61496..61747	+	252	WP_223195197.1	replication protein RepA	-
L6D13_RS24230 (61764)	61764..62021	-	258	WP_000083845.1	replication regulatory protein RepA	-
L6D13_RS24235 (62305)	62305..62454	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (62498)	62498..62559	+	62	NuclAT_1	-	Antitoxin
- (62498)	62498..62559	+	62	NuclAT_1	-	Antitoxin
- (62498)	62498..62559	+	62	NuclAT_1	-	Antitoxin
- (62498)	62498..62559	+	62	NuclAT_1	-	Antitoxin
L6D13_RS24240 (62815)	62815..62889	-	75	Protein_60	endonuclease	-
L6D13_RS24245 (63135)	63135..63347	-	213	WP_001299730.1	ANR family transcriptional regulator	-
L6D13_RS24250 (63483)	63483..64043	-	561	WP_000139315.1	fertility inhibition protein FinO	-
L6D13_RS24255 (64146)	64146..65006	-	861	WP_000704513.1	alpha/beta hydrolase	-
L6D13_RS24260 (65065)	65065..65811	-	747	WP_236932592.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iucA / iucB / iucC / iucD / iutA	1..126021	126021

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T233561 WP_001312851.1 NZ_CP091535:c62454-62305 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T233561 NZ_HG992751:1693927-1694229 [Vibrio sp. B1FIG11]
ATGGCTGAAATAATCTGGACTGAGCCAGCGCTATCCGACCTCAATGATATCGCTGAATACATCGCACTTGAAAATATCGT
AGCTGCAAAACAATTGGTTCAAGCGATCTTTTCTAAAGTCGAGCGCTTAGAGGCTTTCCCTGAATCGGGTCGCATTCCGC
CTGAACTAGAACATCTAAGTTATCGTGAAGTTGTAGTTAATCCGTGCCGTATTTTCTATAAACAAGACGGCGACAAAGTA
TTTATTTTGTTTGTTATGCGTGCTGAGAGAGACTTGCGTAAGTTCCTATTGAGCAAGCAATAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT233561 NZ_CP091535:62498-62559 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin