Detailed information of TA system TA233543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1192638..1193365
• Replicon: chromosome
• Accession: NZ_CP091534
• Organism: Escherichia coli strain 537-20

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: L6D13_RS05765
• Protein ID: WP_000547564.1
• Coordinates: 1192638..1192949 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L6D13_RS05770
• Protein ID: WP_000126294.1
• Coordinates: 1192946..1193365 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6D13_RS05735 (1187780)	1187780..1189489	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
L6D13_RS05740 (1189499)	1189499..1190041	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
L6D13_RS05745 (1190041)	1190041..1190808	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
L6D13_RS05750 (1190805)	1190805..1191215	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
L6D13_RS05755 (1191208)	1191208..1191678	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
L6D13_RS05760 (1191703)	1191703..1192464	+	762	WP_001026446.1	hypothetical protein	-
L6D13_RS05765 (1192638)	1192638..1192949	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
L6D13_RS05770 (1192946)	1192946..1193365	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
L6D13_RS05775 (1193479)	1193479..1194903	-	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
L6D13_RS05780 (1194912)	1194912..1196369	-	1458	WP_236932546.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
L6D13_RS05785 (1196629)	1196629..1197639	+	1011	WP_001363554.1	DNA-binding transcriptional regulator AscG	-
L6D13_RS05790 (1197788)	1197788..1198315	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T233543 WP_000547564.1 NZ_CP091534:1192638-1192949 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T233543 NZ_HG992341:72870-73139 [Xanthomonas arboricola pv. corylina]
GTGGCCGACCTGGACGCGATTGCCGACTACATTGCGCTTGAGGACGCTGCCGCCGCAGCGGCACTGGTGAGACGGGTGTT
TGCGCATGTCGAGCAATTGGCCGAACACCCGGAGAGTGGAAGCCGGCCGCAGGAGCTCAAGCGCTCCCGCTACCGTCAAA
TCGTCGAGCCACCTTGCCGCGTGTTCTACCGTGTGGACGGGCAACGCGTTGTCGTGGTTCACGTGATGCGCTCGGAGCGG
TTACTTCGGAAGAATCGTCTTTCGCGCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT233543 WP_000126294.1 NZ_CP091534:1192946-1193365 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT233543 NZ_HG992341:72595-72852 [Xanthomonas arboricola pv. corylina]
ATGCGCACAGAACTTGTCACCACGCTCAAGCGGCAGGCCACCGAGCTCCTCGCAGATGTCGAGCGCGACAAGGAGCCGAT
CCTGATCACCCAGCATGGCCTGCCCAGCGCATACCTGGTGGACGTCGCCAGCTATGAACGCATGCAACAGCGCATGGCGA
TCCTCGAAGGAATTGCCCGTGGTGAAATGGCGGTAGCCGAAGGCCGCAGCCTGAGCCACGAGCAAGCCAAACAACGCCTG
GCGCGATGGCTGAAATAG

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5