Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 670579..671378 Replicon chromosome
Accession NZ_CP091534
Organism Escherichia coli strain 537-20

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag L6D13_RS03265 Protein ID WP_000347251.1
Coordinates 670579..671043 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag L6D13_RS03270 Protein ID WP_001307405.1
Coordinates 671043..671378 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6D13_RS03235 (665580) 665580..666014 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
L6D13_RS03240 (666032) 666032..666910 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
L6D13_RS03245 (666900) 666900..667679 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
L6D13_RS03250 (667690) 667690..668163 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
L6D13_RS03255 (668186) 668186..669466 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
L6D13_RS03260 (669715) 669715..670524 + 810 WP_000072192.1 aga operon transcriptional regulator AgaR -
L6D13_RS03265 (670579) 670579..671043 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
L6D13_RS03270 (671043) 671043..671378 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
L6D13_RS03275 (671527) 671527..673098 - 1572 WP_001273761.1 galactarate dehydratase -
L6D13_RS03280 (673473) 673473..674807 + 1335 WP_000599653.1 galactarate/glucarate/glycerate transporter GarP -
L6D13_RS03285 (674823) 674823..675593 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T233538 WP_000347251.1 NZ_CP091534:c671043-670579 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T233538 NZ_HG992337:c4434592-4434440 [Xanthomonas arboricola]
ATGAAGCGACTGCTGACACTGATGGTACTGGGCCTGTTTTCGGCCGGCGTGATGACTGGCTGCAACACCATGGCCGGCGC
TGGCAAGGACATGCAGGGTGCAGGTGAGAAGGTCGAAAAGAAGGCCGACAATTGCAGCGACGGTAAGTGCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT233538 WP_001307405.1 NZ_CP091534:c671378-671043 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT233538 NZ_HG992337:c4434357-4434223 [Xanthomonas arboricola]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGGTGTTGTCGGTCGGTATGCTGTCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCTGCGCGCAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References