Detailed information of TA system TA233514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2442536..2443053
• Replicon: chromosome
• Accession: NZ_CP091525
• Organism: Staphylococcus aureus strain N09CSA16

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: L4X60_RS11980
• Protein ID: WP_000113265.1
• Coordinates: 2442787..2443053 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: L4X60_RS11975
• Protein ID: WP_000584499.1
• Coordinates: 2442536..2442787 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L4X60_RS11945 (2438235)	2438235..2438954	+	720	WP_000479576.1	amino acid ABC transporter permease	-
L4X60_RS11950 (2438951)	2438951..2439682	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
L4X60_RS11955 (2439779)	2439779..2441028	-	1250	Protein_2325	FemA/FemB family glycyltransferase FmhA	-
L4X60_RS11960 (2441283)	2441283..2441645	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
L4X60_RS11965 (2441664)	2441664..2442263	+	600	WP_000162810.1	DsbA family protein	-
L4X60_RS11970 (2442291)	2442291..2442371	+	81	WP_223197974.1	hypothetical protein	-
L4X60_RS11975 (2442536)	2442536..2442787	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
L4X60_RS11980 (2442787)	2442787..2443053	+	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
L4X60_RS11985 (2443088)	2443088..2443159	+	72	Protein_2331	hypothetical protein	-
L4X60_RS11990 (2443348)	2443348..2444895	+	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
L4X60_RS11995 (2445081)	2445081..2445548	+	468	WP_000153347.1	SRPBCC domain-containing protein	-
L4X60_RS12000 (2445632)	2445632..2445814	+	183	WP_000230292.1	hypothetical protein	-
L4X60_RS12005 (2446031)	2446031..2446855	+	825	WP_000572038.1	formate/nitrite transporter family protein	-
L4X60_RS12010 (2447089)	2447089..2447625	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T233514 WP_000113265.1 NZ_CP091525:2442787-2443053 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T233514 NZ_HG969191:894971-895240 [Bartonella henselae]
TTGGCTTGGACGATTAGATATGAAAAAAAAGCTCTCAGTTTTTTAAAAAAATGCGATAAAAAAGAAGCACGGCGGATTGT
TGATTTTTTAGATCAACACGTTGCTCCTCTTGAAGATGTGCGTGTAATAGGTAAGCCCTTAAAAGGACAATTCCCAGGTT
TATGGAGATATCGTGTAGGAGATTACAGGATACTTTGTGAACTCTATGATAAGGAGCTTGTCGTATTAGTTTTGGCTGTT
GGACATAGAAAAAATATATATAAAGGTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT233514 WP_000584499.1 NZ_CP091525:2442536-2442787 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT233514 NZ_HG969191:894760-894987 [Bartonella henselae]
ATGACGATATCTATTCGGTTGCCAAGTGATCTCGAAACACGTTTGAATAATTTAGCTGCTAAGACAGGACGTACAAAGTC
TTTTTATTTACGCGAGATTATTGAACGTGGCATAGAGGAAGCAGAGGATTATTATTTAGCCTCACAAGTAAGAGAGCGCG
TTCGAAGGGGAGAGGGTACTTTTTATAGCTCTGAAGAGGTGAGGAAAGAGCTTGGCTTGGACGATTAG

Structures

Toxin

• PDB: 7BWF

Antitoxin

• AlphaFold DB: T1YB60