Detailed information of TA system TA233493

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2425597..2426114
• Replicon: chromosome
• Accession: NZ_CP091523
• Organism: Staphylococcus aureus strain N12HSA28

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: L5471_RS11825
• Protein ID: WP_000113262.1
• Coordinates: 2425848..2426114 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: L5471_RS11820
• Protein ID: WP_000584499.1
• Coordinates: 2425597..2425848 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5471_RS11790 (2421295)	2421295..2422014	+	720	WP_000479576.1	amino acid ABC transporter permease	-
L5471_RS11795 (2422011)	2422011..2422742	+	732	WP_000615458.1	ATP-binding cassette domain-containing protein	-
L5471_RS11800 (2422839)	2422839..2424089	-	1251	WP_001012222.1	FemA/FemB family glycyltransferase FmhA	-
L5471_RS11805 (2424344)	2424344..2424706	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
L5471_RS11810 (2424725)	2424725..2425324	+	600	WP_000162810.1	DsbA family protein	-
L5471_RS11815 (2425352)	2425352..2425432	+	81	WP_223197974.1	hypothetical protein	-
L5471_RS11820 (2425597)	2425597..2425848	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
L5471_RS11825 (2425848)	2425848..2426114	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
L5471_RS11830 (2426149)	2426149..2426220	+	72	WP_223200558.1	hypothetical protein	-
L5471_RS11835 (2426408)	2426408..2427955	+	1548	WP_000727756.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
L5471_RS11840 (2428141)	2428141..2428608	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
L5471_RS11845 (2428692)	2428692..2428874	+	183	WP_000230294.1	hypothetical protein	-
L5471_RS11850 (2429091)	2429091..2429915	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
L5471_RS11855 (2430159)	2430159..2430686	+	528	WP_000973468.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T233493 WP_000113262.1 NZ_CP091523:2425848-2426114 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T233493 NZ_HG941719:c124437-124021 [Escherichia coli O25b:H4-ST131]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT233493 WP_000584499.1 NZ_CP091523:2425597-2425848 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT233493 NZ_HG941719:c124664-124434 [Escherichia coli O25b:H4-ST131]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• AlphaFold DB: T1YB60