Detailed information of TA system TA233479

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-SprF1/-
• Location: 20491..20829
• Replicon: chromosome
• Accession: NZ_CP091523
• Organism: Staphylococcus aureus strain N12HSA28

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: Q2FWU9
• Locus tag: L5471_RS00105
• Protein ID: WP_011447039.1
• Coordinates: 20491..20667 (+)
• Length: 59 a.a.

Antitoxin (RNA)

Gene name	SprF1
Locus tag	-
Coordinates	20655..20829 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5471_RS00085	18321..18473	+	153	WP_001000058.1	hypothetical protein	-
L5471_RS00090	18519..18806	+	288	WP_001262621.1	hypothetical protein	-
L5471_RS00095	18862..19236	+	375	WP_000340977.1	hypothetical protein	-
L5471_RS00100	19609..20382	+	774	WP_000750412.1	staphylococcal enterotoxin type A	-
L5471_RS00105	20491..20667	+	177	WP_011447039.1	type I toxin-antitoxin system toxin PepG1	Toxin
-	20655..20829	-	175	-	-	Antitoxin
L5471_RS00115	20879..21133	+	255	WP_000611512.1	phage holin	-
L5471_RS00120	21145..21900	+	756	WP_000861038.1	CHAP domain-containing protein	-
L5471_RS00125	22091..22582	+	492	WP_249452175.1	staphylokinase	-
L5471_RS00130	23233..23568	+	336	Protein_25	SH3 domain-containing protein	-
L5471_RS00135	24080..24430	+	351	WP_000702263.1	complement inhibitor SCIN-A	-
L5471_RS00140	24483..24743	-	261	WP_001791826.1	hypothetical protein	-
L5471_RS00145	25054..25233	-	180	WP_000669791.1	hypothetical protein	-
L5471_RS00150	25605..25802	-	198	WP_000239610.1	phospholipase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	sea / sak / scn	52..24430	24378

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (30-58)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6849.45 Da
• Isoelectric Point: 10.6777

>T233479 WP_011447039.1 NZ_CP091523:20491-20667 [Staphylococcus aureus]
MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIALIGLVIKLIELSNKK

Nucleotide

• Length: 177 bp

>T233479 NZ_HG941718:3577349-3577813 [Escherichia coli O25b:H4-ST131]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Nucleotide

• Length: 175 bp

>AT233479 NZ_CP091523:c20829-20655 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Structures

Toxin

• PDB: 7NS1

Antitoxin