Detailed information of TA system TA023346

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 4435948..4436445
• Replicon: chromosome
• Accession: NC_011741
• Organism: Escherichia coli IAI1

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: ECIAI1_RS21680
• Protein ID: WP_001173343.1
• Coordinates: 4436272..4436445 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: L4JK92
• Locus tag: ECIAI1_RS21675
• Protein ID: WP_000398619.1
• Coordinates: 4435948..4436244 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECIAI1_RS21650	4432679..4433398	-	720	WP_000611288.1	two-component system response regulator DcuR	-
ECIAI1_RS21655	4433395..4435026	-	1632	WP_001216486.1	sensor histidine kinase	-
ECIAI1_RS21660	4435207..4435437	+	231	WP_000371700.1	(4Fe-4S)-binding protein	-
ECIAI1_RS21665	4435449..4435721	+	273	WP_000405647.1	N-acetyltransferase	-
ECIAI1_RS21675	4435948..4436244	+	297	WP_000398619.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
ECIAI1_RS21680	4436272..4436445	+	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
ECIAI1_RS21685	4436564..4438081	-	1518	WP_001295074.1	lysine--tRNA ligase	-
ECIAI1_RS21690	4438318..4439775	-	1458	WP_000856834.1	dipeptide/tripeptide permease DtpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View HG738867 Escherichia coli str. K-12 substr. MC4100 Genomic island TA020087 4238430..4247780 0.007 View NC_007779 Escherichia coli str. K-12 substr. W3110 Genomic island TA021458 4357262..4366612 0.007 View NC_012759 Escherichia coli BW2952 Genomic island TA023874 4289342..4298692 0.007 View NZ_HG738867 Escherichia coli str. K-12 substr. MC4100 strain K-12 Genomic island TA285032 4238430..4247780 0.007	inside	Genomic island	-	-	4435948..4445299	9351

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T23346 WP_001173343.1 NC_011741:4436272-4436445 [Escherichia coli IAI1]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T23346 NC_011741:4436272-4436445 [Escherichia coli IAI1]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11467.97 Da
• Isoelectric Point: 4.1705

>AT23346 WP_000398619.1 NC_011741:4435948-4436244 [Escherichia coli IAI1]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT23346 NC_011741:4435948-4436244 [Escherichia coli IAI1]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A7U9QI41

Antitoxin

• AlphaFold DB: A0A7U9QBH6