Detailed information of TA system TA233457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1305907..1306558
• Replicon: chromosome
• Accession: NZ_CP091512
• Organism: Vitreoscilla stercoraria strain SAG 1488-6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LVJ81_RS06420
• Protein ID: WP_019959502.1
• Coordinates: 1305907..1306254 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LVJ81_RS06425
• Protein ID: WP_019959501.1
• Coordinates: 1306256..1306558 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ81_RS06395 (LVJ81_06395)	1302397..1302750	-	354	WP_019957863.1	hypothetical protein	-
LVJ81_RS06400 (LVJ81_06400)	1302782..1303609	-	828	WP_169708866.1	zonular occludens toxin domain-containing protein	-
LVJ81_RS06405 (LVJ81_06405)	1303713..1304489	-	777	WP_244802741.1	IS21-like element helper ATPase IstB	-
LVJ81_RS06410 (LVJ81_06410)	1304486..1305502	-	1017	WP_026353722.1	IS21 family transposase	-
LVJ81_RS06415 (LVJ81_06415)	1305555..1305845	+	291	WP_280520946.1	recombinase family protein	-
LVJ81_RS06420 (LVJ81_06420)	1305907..1306254	+	348	WP_019959502.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LVJ81_RS06425 (LVJ81_06425)	1306256..1306558	+	303	WP_019959501.1	XRE family transcriptional regulator	Antitoxin
LVJ81_RS06430 (LVJ81_06430)	1306843..1308246	-	1404	WP_019959500.1	hypothetical protein	-
LVJ81_RS06435 (LVJ81_06435)	1308575..1309807	-	1233	WP_019959499.1	type II toxin-antitoxin system HipA family toxin	-
LVJ81_RS06440 (LVJ81_06440)	1309804..1310124	-	321	WP_040755970.1	helix-turn-helix transcriptional regulator	-
LVJ81_RS06445 (LVJ81_06445)	1310407..1311009	+	603	WP_019959497.1	recombinase family protein	-
LVJ81_RS06450 (LVJ81_06450)	1311083..1311385	-	303	WP_019959496.1	HigA family addiction module antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1292991..1420048	127057
	-	flank	IS/Tn	-	-	1303713..1304489	776

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (30-81)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13212.17 Da
• Isoelectric Point: 6.2293

>T233457 WP_019959502.1 NZ_CP091512:1305907-1306254 [Vitreoscilla stercoraria]
MWTVITTNLFNEWLDEQDESTQEKVLAGLVVLQQQGPSLGRPLVDTVYGSKFTNMKELRIQHKGKPLRAFFAFDPLRQAI
VLCVGDKSGKKRFYIEMIAIADEEYERHLSTLGEH

Nucleotide

• Length: 348 bp

>T233457 NZ_HG938371:c798244-797945 [Burkholderia cenocepacia H111]
ATGGACAAGATCAAGACCCGGCCGTGGGATTCGGCCGAACACCTGAAAACCGAAGACGACATGGCCGACTACTTCGACGC
ATGCCTGCAGGAGGCCGGCGACGATCCGGCCTTCATCGCGCATGCACTCGGCGTGATCGCCCGCGCACGCGGCATGTCGC
AGGTCGCGCGCGATGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAAGTCATCAAGGCGCTTGGCCTGCAATTGCACGGCGCCAAGGCTGCCGTGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11151.91 Da
• Isoelectric Point: 5.1881

>AT233457 WP_019959501.1 NZ_CP091512:1306256-1306558 [Vitreoscilla stercoraria]
MAKTLDELLASRSPESQARIQEMADELLLANQLYLIREELEISQKQLAEALGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT233457 NZ_HG938371:c798565-798248 [Burkholderia cenocepacia H111]
ATGCCCTACAGTCCTCCTGCATTCAGCATCCGGACCACCGACGTCTTCGACGCCTGGTTTGCCGGCCTGCCCGATCGCCT
CGCCAAACGCCGCATCCAGGCGCGCATCGACCGCCTGTCGATGGGCAATCCGGGCGACTGGAAATCCGCTGGCTCCCCCG
TCGTCGAAATGCGTATCGACCACGGCCCGGGCTATCGCGTCTATTACGTGAGGCGCGGAACGATATGGGTCATCCTGCTG
TGCGGCGGCGATAAATCGACGCAACAGGCCGACATCCGCGCCGCGCACGCAATGCTCGCGCACCTCGACATGGAGTGA