Detailed information of TA system TA023345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4159399..4160001
• Replicon: chromosome
• Accession: NC_011741
• Organism: Escherichia coli IAI1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: ECIAI1_RS20355
• Protein ID: WP_000897305.1
• Coordinates: 4159399..4159710 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECIAI1_RS20360
• Protein ID: WP_000356397.1
• Coordinates: 4159711..4160001 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECIAI1_RS20325	4154429..4155214	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
ECIAI1_RS20330	4155313..4155912	+	600	WP_001295269.1	glucose-1-phosphatase	-
ECIAI1_RS20335	4155906..4156778	+	873	WP_000920762.1	virulence factor BrkB family protein	-
ECIAI1_RS20340	4156775..4157212	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ECIAI1_RS20345	4157257..4158198	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
ECIAI1_RS20350	4158262..4159170	-	909	WP_001162704.1	alpha/beta hydrolase	-
ECIAI1_RS20355	4159399..4159710	+	312	WP_000897305.1	hypothetical protein	Toxin
ECIAI1_RS20360	4159711..4160001	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
ECIAI1_RS20365	4160606..4160824	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
ECIAI1_RS20370	4161043..4161285	+	243	WP_001086388.1	hypothetical protein	-
ECIAI1_RS20380	4161615..4162544	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
ECIAI1_RS20385	4162541..4163176	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
ECIAI1_RS20390	4163173..4164075	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T23345 WP_000897305.1 NC_011741:4159399-4159710 [Escherichia coli IAI1]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T23345 NC_011741:4159399-4159710 [Escherichia coli IAI1]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT23345 WP_000356397.1 NC_011741:4159711-4160001 [Escherichia coli IAI1]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT23345 NC_011741:4159711-4160001 [Escherichia coli IAI1]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7