Detailed information of TA system TA023343

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3363244..3364043
• Replicon: chromosome
• Accession: NC_011741
• Organism: Escherichia coli IAI1

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: U9XVR9
• Locus tag: ECIAI1_RS16405
• Protein ID: WP_000347267.1
• Coordinates: 3363579..3364043 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: ECIAI1_RS16400
• Protein ID: WP_001307405.1
• Coordinates: 3363244..3363579 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECIAI1_RS16380	3359029..3359799	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
ECIAI1_RS16385	3359815..3361149	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
ECIAI1_RS16395	3361524..3363095	+	1572	WP_001273741.1	galactarate dehydratase	-
ECIAI1_RS16400	3363244..3363579	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
ECIAI1_RS16405	3363579..3364043	+	465	WP_000347267.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
ECIAI1_RS16410	3364098..3364907	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
ECIAI1_RS16415	3365156..3366436	+	1281	WP_000681936.1	tagatose-bisphosphate aldolase subunit KbaZ	-
ECIAI1_RS16420	3366459..3366932	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
ECIAI1_RS16425	3366943..3367722	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
ECIAI1_RS16430	3367712..3368590	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
ECIAI1_RS16435	3368608..3369042	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3352588..3364043	11455

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17807.17 Da
• Isoelectric Point: 9.4947

>T23343 WP_000347267.1 NC_011741:3363579-3364043 [Escherichia coli IAI1]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T23343 NC_011741:3363579-3364043 [Escherichia coli IAI1]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT23343 WP_001307405.1 NC_011741:3363244-3363579 [Escherichia coli IAI1]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT23343 NC_011741:3363244-3363579 [Escherichia coli IAI1]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0XTR4

Antitoxin

• AlphaFold DB: A0A0E0XVC7