Detailed information of TA system TA233426

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3625524..3626181
• Replicon: chromosome
• Accession: NZ_CP091492
• Organism: Enterobacter cloacae complex sp. ECL405

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A808IF03
• Locus tag: L3070_RS17305
• Protein ID: WP_033146338.1
• Coordinates: 3625524..3625823 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L3070_RS17310
• Protein ID: WP_094935697.1
• Coordinates: 3625834..3626181 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3070_RS17295 (L3070_17295)	3621894..3624092	+	2199	WP_112778387.1	type I secretion system permease/ATPase	-
L3070_RS17300 (L3070_17300)	3624103..3625272	+	1170	WP_086374946.1	HlyD family efflux transporter periplasmic adaptor subunit	-
L3070_RS17305 (L3070_17305)	3625524..3625823	+	300	WP_033146338.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L3070_RS17310 (L3070_17310)	3625834..3626181	+	348	WP_094935697.1	HigA family addiction module antitoxin	Antitoxin
L3070_RS17315 (L3070_17315)	3626226..3626651	+	426	WP_033146340.1	nucleoside triphosphatase NudI	-
L3070_RS17320 (L3070_17320)	3626623..3627354	-	732	WP_094935696.1	helix-turn-helix transcriptional regulator	-
L3070_RS17325 (L3070_17325)	3627452..3627871	+	420	WP_039263434.1	nuclear transport factor 2 family protein	-
L3070_RS17330 (L3070_17330)	3627874..3628392	-	519	WP_094935695.1	hypothetical protein	-
L3070_RS17335 (L3070_17335)	3628477..3629658	-	1182	WP_094935694.1	PLP-dependent aminotransferase family protein	-
L3070_RS17340 (L3070_17340)	3629679..3630566	-	888	WP_094935693.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-95)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (8-86)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11691.32 Da
• Isoelectric Point: 10.0346

>T233426 WP_033146338.1 NZ_CP091492:3625524-3625823 [Enterobacter cloacae complex sp. ECL405]
MISYFRDQWLEDFFLYGRSSNVIPANLETALARKLDIIRAATSHRDLRSPPGNMYEALNPPLKGYSSIRVNRQYRLVFRW
TEGKAEDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T233426 NZ_HG938354:c172944-172573 [Neorhizobium galegae bv. orientalis str. HAMBI 540]
ATGATCCACAGCAACGTGCCGAGGCGCGGCGATGTCTACTGGATCGACCCAAACCCGGTCGCCGGCCGGGAAATGAAGAA
CCGGCATCGTTTCGTCGTCATCACGCCGATCGAGATCAACAGGCTGGGAGTCAGCATGACGGTGCCCATCACCACCGGTG
GGGCTTTTATCCGCGATGTCGGTCTTGCGGTGGCGATTTCCGGGCACGACACGACCGGTGTCGCCGTTTGCAACCAGGTC
CGCAGCTTCGACATTCCCGCCCGCATCCAGCAGAAAACCGCGCAATATATCGAGACGCTGGACGAGGCGACCATGAATGA
AATCGTCTCGCGCGTGGTCAGCGCCATCGACCCTGCACCTGAACCCGCCTGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13084.05 Da
• Isoelectric Point: 7.0134

>AT233426 WP_094935697.1 NZ_CP091492:3625834-3626181 [Enterobacter cloacae complex sp. ECL405]
MTLQQALRKPTTPGDVMQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAIKLAKAFDTTIEFWLNLQLNVD
IWEAQSNPRTQEELSRIKTVADVMAKRKSGQPDVA

Nucleotide

• Length: 348 bp

>AT233426 NZ_HG938354:c173207-172944 [Neorhizobium galegae bv. orientalis str. HAMBI 540]
ATGCCGGTCACCACCAAAATCCGACGCCAAGGCGGTGCTGCGGTGATTACCATCCCGCCGGCCCTGTTGAAGCTGATGCA
TACCGAAGTCGGCGATCAACTCACACTCGACGTCGCCAACGGTGAATTGATCGCCCGTCCCGTCGAGAGCGGCCGCAAGC
GCTATACCCTTAGCGAATTGCTGAAGGGAAGCGATGCCATGGCGATGCTGAATGCCGCGGTCGCCGATGCGCAGGAAGGC
GATCCGATAGGGCACGAGATCTGA

Structures

Toxin

• AlphaFold DB: A0A808IF03