Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3019694..3020354 Replicon chromosome
Accession NZ_CP091492
Organism Enterobacter cloacae complex sp. ECL405

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag L3070_RS14615 Protein ID WP_058841455.1
Coordinates 3020001..3020354 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag L3070_RS14610 Protein ID WP_058841454.1
Coordinates 3019694..3019996 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3070_RS14590 (L3070_14590) 3015672..3017861 + 2190 WP_112778179.1 TonB-dependent siderophore receptor -
L3070_RS14600 (L3070_14600) 3018156..3018557 - 402 WP_112778178.1 RidA family protein -
L3070_RS14605 (L3070_14605) 3018699..3019619 + 921 WP_126327674.1 LysR family transcriptional regulator -
L3070_RS14610 (L3070_14610) 3019694..3019996 - 303 WP_058841454.1 XRE family transcriptional regulator Antitoxin
L3070_RS14615 (L3070_14615) 3020001..3020354 - 354 WP_058841455.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L3070_RS14620 (L3070_14620) 3020532..3021425 - 894 WP_112778177.1 LysR substrate-binding domain-containing protein -
L3070_RS14625 (L3070_14625) 3021599..3022204 + 606 WP_048980132.1 glutathione S-transferase family protein -
L3070_RS14630 (L3070_14630) 3022248..3023198 - 951 WP_023336219.1 HTH-type transcriptional regulator Cbl -
L3070_RS14635 (L3070_14635) 3023295..3024212 - 918 WP_008499475.1 nitrogen assimilation transcriptional regulator NAC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-106)

Antitoxin

HigA2-like, helix-turn-helix domain

(25-89)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13522.32 Da        Isoelectric Point: 7.1678

>T233424 WP_058841455.1 NZ_CP091492:c3020354-3020001 [Enterobacter cloacae complex sp. ECL405]
VWAINTTDRFDRWFTSLDDTDRASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
MLCAGNKVGNERRFYDEMLLVADQEFTHWLDTLKERE

Download         Length: 354 bp

>T233424 NZ_HG938353:c2823672-2823394 [Neorhizobium galegae bv. orientalis str. HAMBI 540]
ATGATCAAATCGTGGGTCAATAGTTCAACCCGTCGCTTTGCCGAGGAAGGCAAATCCAAGTTTTCGGGGATGAACGAAGA
GGCCTCGATCGAGTTGTTGGCGGCGCTCCACGCAGCCTCGTCCTTGAACGATCTCAGTCCACTGAAATCGGTCGGCCTTC
ACAAGCTTTCCGGAAATCGCTTAGGGCAGTGGGCAATGACGATCAACGGGCCGTGGCGGATCTGTTTCACGTTCAGGGAT
GGAGATGACTGGGACGTGGAAATCGTCGACTATCATTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11016.82 Da        Isoelectric Point: 5.7316

>AT233424 WP_058841454.1 NZ_CP091492:c3019996-3019694 [Enterobacter cloacae complex sp. ECL405]
MGRTLEQLIADEKPEVVASAQAIATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYELVL

Download         Length: 303 bp

>AT233424 NZ_HG938353:c2823380-2823096 [Neorhizobium galegae bv. orientalis str. HAMBI 540]
ATGATAGCAGTTCATCCGGGTCGCATTTTGAAGCGCGAACTCACCGCACGCAGCCTGTCGGCCAATCAGCTCGCCCTTTC
ACTTCGCCTGCCATCTGGCCGCATCACCGACATTCTCAACGCAAAGCGCGGCATCTCGCCCGATACAGCCCTGAGATTGG
CACGATATTTTGGAAACAGCGCCCGCTTTTGGCTCGATCTGCAGACCGCATATGAACTCGCTCTTGCGGAACGAGAAAAT
GGCGAACGGATCGCGGAAGAGGTCAAACCGGCCGAAGCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References