Detailed information of TA system TA233392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4749828..4750430
• Replicon: chromosome
• Accession: NZ_CP091481
• Organism: Enterobacter cloacae complex sp. ECL414

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: L3068_RS22890
• Protein ID: WP_117582088.1
• Coordinates: 4750119..4750430 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8B2XUJ3
• Locus tag: L3068_RS22885
• Protein ID: WP_032635182.1
• Coordinates: 4749828..4750118 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3068_RS22870 (L3068_22870)	4747326..4748228	+	903	WP_008501831.1	formate dehydrogenase subunit beta	-
L3068_RS22875 (L3068_22875)	4748225..4748860	+	636	WP_008501832.1	formate dehydrogenase cytochrome b556 subunit	-
L3068_RS22880 (L3068_22880)	4748857..4749786	+	930	WP_014885738.1	formate dehydrogenase accessory protein FdhE	-
L3068_RS22885 (L3068_22885)	4749828..4750118	-	291	WP_032635182.1	NadS family protein	Antitoxin
L3068_RS22890 (L3068_22890)	4750119..4750430	-	312	WP_117582088.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L3068_RS22895 (L3068_22895)	4750659..4751579	+	921	WP_117582090.1	alpha/beta hydrolase	-
L3068_RS22900 (L3068_22900)	4751588..4752529	-	942	WP_023331628.1	fatty acid biosynthesis protein FabY	-
L3068_RS22905 (L3068_22905)	4752574..4753011	-	438	WP_014885747.1	D-aminoacyl-tRNA deacylase	-
L3068_RS22910 (L3068_22910)	4753008..4753889	-	882	WP_014885748.1	virulence factor BrkB family protein	-
L3068_RS22915 (L3068_22915)	4753883..4754482	-	600	WP_023331629.1	glucose-1-phosphatase	-
L3068_RS22920 (L3068_22920)	4754602..4755402	-	801	WP_014885750.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12236.26 Da
• Isoelectric Point: 10.1230

>T233392 WP_117582088.1 NZ_CP091481:c4750430-4750119 [Enterobacter cloacae complex sp. ECL414]
MLFIETDIFTEDVKTLLDDDEYHKLQVFLATQPNYGDLIQNTGGLRKIRWFSGSKGKRGGVRVIYFHRTREFEIRLLLIY
RKGIKDDLSAKEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T233392 NZ_HG813250:52022-52363 [Listeria monocytogenes 6179]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10703.22 Da
• Isoelectric Point: 10.5888

>AT233392 WP_032635182.1 NZ_CP091481:c4750118-4749828 [Enterobacter cloacae complex sp. ECL414]
MDKALFERLTQSIAQMNEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALSQ

Nucleotide

• Length: 291 bp

>AT233392 NZ_HG813250:51786-52028 [Listeria monocytogenes 6179]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAA

Structures

Antitoxin

• AlphaFold DB: A0A8B2XUJ3