Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2139428..2139930 Replicon chromosome
Accession NC_011741
Organism Escherichia coli IAI1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ECIAI1_RS10550 Protein ID WP_000767829.1
Coordinates 2139428..2139682 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ECIAI1_RS10555 Protein ID WP_001259255.1
Coordinates 2139679..2139930 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECIAI1_RS10525 2134443..2134670 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
ECIAI1_RS10530 2134684..2135742 - 1059 WP_000492335.1 transport protein YeeE -
ECIAI1_RS10535 2135921..2137279 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ECIAI1_RS25165 2137269..2137331 - 63 WP_010723108.1 membrane protein YoeI -
ECIAI1_RS10540 2137546..2138475 - 930 WP_000803351.1 LysR family transcriptional regulator -
ECIAI1_RS10545 2138521..2139345 - 825 WP_000754763.1 SDR family oxidoreductase -
ECIAI1_RS10550 2139428..2139682 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ECIAI1_RS10555 2139679..2139930 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ECIAI1_RS23770 2140213..2140263 + 51 WP_001364200.1 his operon leader peptide -
ECIAI1_RS10560 2140409..2141308 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ECIAI1_RS10565 2141314..2142618 + 1305 WP_001317939.1 histidinol dehydrogenase -
ECIAI1_RS10570 2142615..2143685 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
ECIAI1_RS10575 2143685..2144752 + 1068 WP_000080060.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T23339 WP_000767829.1 NC_011741:c2139682-2139428 [Escherichia coli IAI1]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T23339 NC_011741:c2139682-2139428 [Escherichia coli IAI1]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT23339 WP_001259255.1 NC_011741:c2139930-2139679 [Escherichia coli IAI1]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT23339 NC_011741:c2139930-2139679 [Escherichia coli IAI1]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References