Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1171031..1171680 Replicon chromosome
Accession NZ_CP091481
Organism Enterobacter cloacae complex sp. ECL414

Toxin (Protein)


Gene name higB Uniprot ID A0A2W0G579
Locus tag L3068_RS05385 Protein ID WP_045281668.1
Coordinates 1171318..1171680 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2W0GCS7
Locus tag L3068_RS05380 Protein ID WP_014882863.1
Coordinates 1171031..1171330 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3068_RS05370 (L3068_05370) 1167456..1169429 + 1974 WP_117581116.1 PhoX family phosphatase -
L3068_RS05375 (L3068_05375) 1169532..1170920 + 1389 WP_014882862.1 phenylalanine transporter -
L3068_RS05380 (L3068_05380) 1171031..1171330 - 300 WP_014882863.1 helix-turn-helix transcriptional regulator Antitoxin
L3068_RS05385 (L3068_05385) 1171318..1171680 - 363 WP_045281668.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L3068_RS05390 (L3068_05390) 1171886..1173121 + 1236 WP_023332295.1 MFS transporter -
L3068_RS05395 (L3068_05395) 1173137..1174162 + 1026 WP_032637265.1 sugar phosphate isomerase/epimerase -
L3068_RS05400 (L3068_05400) 1174155..1175297 + 1143 WP_117581114.1 Gfo/Idh/MocA family oxidoreductase -
L3068_RS05405 (L3068_05405) 1175373..1176344 + 972 WP_014882868.1 LacI family DNA-binding transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-80)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13910.87 Da        Isoelectric Point: 5.6482

>T233380 WP_045281668.1 NZ_CP091481:c1171680-1171318 [Enterobacter cloacae complex sp. ECL414]
MWNVETTDIFDQWYFEQTTALKEDVLAMMHILAEFGPTLGRPYVDTVKASAYANMKELRIQHAGNPIRAFFAFDPDRKAI
VLCAGDKTGCNQKRFYNEMITLADVEYSRHLADKEAIWQR

Download         Length: 363 bp

>T233380 NZ_CP091481:c1171680-1171318 [Enterobacter cloacae complex sp. ECL414]
ATGTGGAATGTCGAGACAACGGACATATTTGATCAATGGTACTTCGAACAAACAACAGCATTAAAAGAAGATGTGCTAGC
GATGATGCATATTCTTGCTGAGTTTGGCCCAACGCTTGGGCGGCCATACGTAGATACCGTGAAGGCTTCAGCCTATGCGA
ACATGAAGGAATTACGGATTCAACATGCGGGAAATCCCATCAGAGCTTTTTTTGCTTTTGATCCTGACCGTAAGGCAATT
GTGCTCTGTGCTGGAGACAAAACCGGTTGCAATCAAAAACGGTTTTATAACGAAATGATTACGCTGGCTGACGTTGAGTA
CAGCAGACATCTGGCGGATAAGGAGGCCATATGGCAACGCTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10860.61 Da        Isoelectric Point: 9.8627

>AT233380 WP_014882863.1 NZ_CP091481:c1171330-1171031 [Enterobacter cloacae complex sp. ECL414]
MATLKELMAQQSAESQERIAAKVDVIRRIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GEMSIDVTLPNGKRIAFQI

Download         Length: 300 bp

>AT233380 NZ_CP091481:c1171330-1171031 [Enterobacter cloacae complex sp. ECL414]
ATGGCAACGCTAAAAGAACTCATGGCACAACAGAGTGCGGAAAGTCAGGAGCGTATTGCGGCTAAAGTTGATGTAATACG
CAGAATTGTTGCCCTGAACCAACTGCGGGAAGAGCTCAATATTTCGCAGACAGAACTGGCCGCGGCAATGGGGGTAAAAC
AACCAACTGTGGCAAAAATCGAACAGCCGGGAAACGATCCCCGGCTTTCAACACTCAAACGCTATGTCAGTGCGCTCGGC
GGTGAAATGAGCATTGATGTGACGTTACCGAATGGGAAACGAATAGCGTTTCAGATTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W0G579

Antitoxin

Source ID Structure
AlphaFold DB A0A2W0GCS7

References