Detailed information of TA system TA233376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 31815..32545
• Replicon: chromosome
• Accession: NZ_CP091481
• Organism: Enterobacter cloacae complex sp. ECL414

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2W0GI63
• Locus tag: L3068_RS00150
• Protein ID: WP_023331686.1
• Coordinates: 31815..32129 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L3068_RS00155
• Protein ID: WP_023331687.1
• Coordinates: 32126..32545 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3068_RS00130 (L3068_00130)	27480..28568	+	1089	WP_023331681.1	cellulase family glycosylhydrolase	-
L3068_RS00135 (L3068_00135)	28471..29655	-	1185	WP_045269345.1	multidrug efflux MFS transporter EmrD	-
L3068_RS00140 (L3068_00140)	29831..30664	-	834	WP_117582104.1	EamA family transporter	-
L3068_RS00145 (L3068_00145)	30727..31173	-	447	WP_014881927.1	GNAT family N-acetyltransferase	-
L3068_RS00150 (L3068_00150)	31815..32129	+	315	WP_023331686.1	type II toxin-antitoxin system HigB family toxin	Toxin
L3068_RS00155 (L3068_00155)	32126..32545	+	420	WP_023331687.1	helix-turn-helix domain-containing protein	Antitoxin
L3068_RS00160 (L3068_00160)	32695..32793	+	99	WP_023331688.1	ilvB operon leader peptide IvbL	-
L3068_RS00165 (L3068_00165)	32900..34588	+	1689	WP_117582102.1	acetolactate synthase large subunit	-
L3068_RS00170 (L3068_00170)	34592..34879	+	288	WP_010426528.1	acetolactate synthase small subunit	-
L3068_RS00175 (L3068_00175)	34962..35555	+	594	WP_014881931.1	transcriptional regulator UhpA	-
L3068_RS00180 (L3068_00180)	35552..37057	+	1506	WP_045134486.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12441.59 Da
• Isoelectric Point: 10.2825

>T233376 WP_023331686.1 NZ_CP091481:31815-32129 [Enterobacter cloacae complex sp. ECL414]
MHLISMKAILDAVIQFPQHREELLFLGRTIEKSHCTTPVALRKIFPTLDNLKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T233376 NZ_HG813240:c2970982-2970659 [Mycobacterium tuberculosis 49-02]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15561.18 Da
• Isoelectric Point: 5.6761

>AT233376 WP_023331687.1 NZ_CP091481:32126-32545 [Enterobacter cloacae complex sp. ECL414]
MMIVADAMKATHALVAAVPLLGEHPNEKDYQDALELVEYLLMNEPGSPLLDIVCARIRRYEANRPEIVALRQEMESVPVG
IAVLRTLMDQYKLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT233376 NZ_HG813240:c2971307-2971062 [Mycobacterium tuberculosis 49-02]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Structures

Toxin

• AlphaFold DB: A0A2W0GI63