Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 40279..40929 Replicon chromosome
Accession NZ_CP091470
Organism Klebsiella michiganensis strain KO_408

Toxin (Protein)


Gene name higB Uniprot ID A0A0H3H3K0
Locus tag L3249_RS00195 Protein ID WP_014227314.1
Coordinates 40279..40620 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H3H3Q5
Locus tag L3249_RS00200 Protein ID WP_014227313.1
Coordinates 40630..40929 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3249_RS00180 (L3249_00180) 36542..37861 + 1320 WP_032751824.1 MFS transporter -
L3249_RS00185 (L3249_00185) 38007..39398 + 1392 WP_004126843.1 hexose-6-phosphate:phosphate antiporter -
L3249_RS00190 (L3249_00190) 39576..40028 + 453 WP_032718748.1 DUF1198 domain-containing protein -
L3249_RS00195 (L3249_00195) 40279..40620 + 342 WP_014227314.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L3249_RS00200 (L3249_00200) 40630..40929 + 300 WP_014227313.1 helix-turn-helix transcriptional regulator Antitoxin
L3249_RS00205 (L3249_00205) 41048..42229 + 1182 WP_014227312.1 purine ribonucleoside efflux pump NepI -
L3249_RS00210 (L3249_00210) 42262..43071 - 810 WP_195378326.1 phosphonoacetaldehyde hydrolase -
L3249_RS00215 (L3249_00215) 43081..44184 - 1104 WP_014227310.1 2-aminoethylphosphonate--pyruvate transaminase -
L3249_RS00220 (L3249_00220) 44325..45044 + 720 WP_195378325.1 phosphonate utilization transcriptional regulator PhnR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13110.00 Da        Isoelectric Point: 5.7545

>T233358 WP_014227314.1 NZ_CP091470:40279-40620 [Klebsiella michiganensis]
MWDVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPRLGRPFADTVNDSAFSNMKELRIQHQGRPIRAFFVFDPSRCGI
VLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Download         Length: 342 bp

>T233358 NZ_HG813240:c1941525-1941136 [Mycobacterium tuberculosis 49-02]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10966.87 Da        Isoelectric Point: 9.7256

>AT233358 WP_014227313.1 NZ_CP091470:40630-40929 [Klebsiella michiganensis]
MATLKELMAKQSPESQARIAEKVEEMRQAIALSMLREELNMSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPTGKRIAFHL

Download         Length: 300 bp

>AT233358 NZ_HG813240:c1941749-1941522 [Mycobacterium tuberculosis 49-02]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H3K0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3Q5

References