Detailed information of TA system TA233331

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 100197..100430
• Replicon: plasmid pMHW-2
• Accession: NZ_CP091428
• Organism: Escherichia coli strain 1000C-3

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: L3Z43_RS23850
• Protein ID: WP_001372321.1
• Coordinates: 100197..100322 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	100399..100430 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3Z43_RS23815 (95577)	95577..96266	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
L3Z43_RS23820 (96453)	96453..96836	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
L3Z43_RS23825 (97154)	97154..97756	+	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
L3Z43_RS23830 (98053)	98053..98874	-	822	WP_001234426.1	DUF932 domain-containing protein	-
L3Z43_RS23835 (98992)	98992..99279	-	288	WP_000107535.1	hypothetical protein	-
L3Z43_RS23840 (99579)	99579..99747	+	169	Protein_118	hypothetical protein	-
L3Z43_RS23845 (99747)	99747..99977	-	231	WP_071886920.1	hypothetical protein	-
L3Z43_RS23850 (100197)	100197..100322	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
L3Z43_RS23855 (100264)	100264..100413	-	150	Protein_121	plasmid maintenance protein Mok	-
- (100399)	100399..100430	-	32	NuclAT_1	-	Antitoxin
- (100399)	100399..100430	-	32	NuclAT_1	-	Antitoxin
- (100399)	100399..100430	-	32	NuclAT_1	-	Antitoxin
- (100399)	100399..100430	-	32	NuclAT_1	-	Antitoxin
L3Z43_RS23860 (100455)	100455..101818	-	1364	Protein_122	IS3-like element IS150 family transposase	-
- (101866)	101866..102063	-	198	NuclAT_0	-	-
- (101866)	101866..102063	-	198	NuclAT_0	-	-
- (101866)	101866..102063	-	198	NuclAT_0	-	-
- (101866)	101866..102063	-	198	NuclAT_0	-	-
L3Z43_RS23865 (101875)	101875..102063	+	189	WP_001299721.1	hypothetical protein	-
L3Z43_RS23870 (102032)	102032..102794	-	763	Protein_124	plasmid SOS inhibition protein A	-
L3Z43_RS23875 (102791)	102791..103225	-	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
L3Z43_RS23880 (103280)	103280..103477	-	198	Protein_126	hypothetical protein	-
L3Z43_RS23885 (103505)	103505..103738	-	234	WP_000006030.1	DUF905 family protein	-
L3Z43_RS23890 (103800)	103800..104297	-	498	WP_061310956.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..145445	145445

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T233331 WP_001372321.1 NZ_CP091428:c100322-100197 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T233331 NZ_HG813240:73372-73773 [Mycobacterium tuberculosis 49-02]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGTGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGTCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAACGTCACGTTCTACGACGCCCTTTACGTCGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin

Nucleotide

• Length: 32 bp

>AT233331 NZ_CP091428:c100430-100399 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin