Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3997908..3998707 Replicon chromosome
Accession NZ_CP091427
Organism Escherichia coli strain 1000C-3

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag L3Z43_RS19140 Protein ID WP_000347275.1
Coordinates 3998243..3998707 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag L3Z43_RS19135 Protein ID WP_001307405.1
Coordinates 3997908..3998243 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3Z43_RS19120 (3993693) 3993693..3994463 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
L3Z43_RS19125 (3994479) 3994479..3995813 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
L3Z43_RS19130 (3996188) 3996188..3997759 + 1572 WP_001273752.1 galactarate dehydratase -
L3Z43_RS19135 (3997908) 3997908..3998243 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
L3Z43_RS19140 (3998243) 3998243..3998707 + 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
L3Z43_RS19145 (3998762) 3998762..3999571 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
L3Z43_RS19150 (3999820) 3999820..4001100 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
L3Z43_RS19155 (4001123) 4001123..4001596 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
L3Z43_RS19160 (4001607) 4001607..4002386 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
L3Z43_RS19165 (4002376) 4002376..4003254 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
L3Z43_RS19170 (4003272) 4003272..4003706 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T233326 WP_000347275.1 NZ_CP091427:3998243-3998707 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T233326 NZ_HG738868:c3344155-3343802 [Serratia marcescens SMB2099]
GTGTGGGAAATTAAAACAACGGATGCGTTTGATAACTGGTTTAGCTCACTCCACGATGCCGATCGGGCTGGCGTGCTTGC
CGCATTGATGATCCTGCGGGAAAAGGGGCCGGGGCTGCCCAGACCCTATGCCGATACGGTTAAAGGTTCGCGTTACAGCA
ATATGAAAGAGCTGCGCATTCAGAGCCGTGGCGAACCGTTGCGGGCATTCTTCGCCTTCGATCCGTACCGTATCGGCATT
GTACTTTGCGCGGGCAATAAAGCCGGTAATGAAAAACGTTTTTACGATCGGATGATTCAGGCTGCCGATCGGGAATTCAC
GAACTGGCTGAACACATTAAACGAGAAGGAGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT233326 WP_001307405.1 NZ_CP091427:3997908-3998243 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT233326 NZ_HG738868:c3343797-3343495 [Serratia marcescens SMB2099]
ATGGGCAGAACGCTGGAACAGCTTATTGCGGATGAAAAGCCCGAGGTCGTGGCCAAAGCGCAAGCGCTGGCGGCTGACAT
CATGCTGAACATCCACCTTGCCGAGCTGCGCGAGAAGGTGCAGAAAACGCAGGTTGAAATGGCGCAGGCGCTGGGTATCC
GGCAACCGACCGTTGCCGTGATGGAAAAACCGGGCCGCGATCTGAAACTGTCTTCGCTCAAACGCTATGTTGAGGCGGCC
GGCGGCAAGCTGCGCCTGGATGTTGAACTGCCAGACGGCTCTCACTACGAATTTGTGCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References