Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3952519..3953246 Replicon chromosome
Accession NZ_CP091427
Organism Escherichia coli strain 1000C-3

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag L3Z43_RS18900 Protein ID WP_000550189.1
Coordinates 3952932..3953246 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag L3Z43_RS18895 Protein ID WP_000560266.1
Coordinates 3952519..3952935 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3Z43_RS18885 (3947679) 3947679..3950030 + 2352 WP_000695487.1 alpha-glucosidase -
L3Z43_RS18890 (3950456) 3950456..3952474 + 2019 WP_000121435.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
L3Z43_RS18895 (3952519) 3952519..3952935 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
L3Z43_RS18900 (3952932) 3952932..3953246 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
L3Z43_RS18905 (3953530) 3953530..3954666 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
L3Z43_RS18910 (3954751) 3954751..3955254 + 504 WP_001333820.1 M48 family metallopeptidase -
L3Z43_RS18915 (3955331) 3955331..3956023 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
L3Z43_RS18920 (3956102) 3956102..3957088 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T233325 WP_000550189.1 NZ_CP091427:c3953246-3952932 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T233325 NZ_HG738868:2043008-2043104 [Serratia marcescens SMB2099]
AACAAGCCCTGCATTAAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGAATATAGGAATC
GTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT233325 WP_000560266.1 NZ_CP091427:c3952935-3952519 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT233325 NZ_HG738868:c2043104-2042964 [Serratia marcescens SMB2099]
AAAAAAAGACCGAATACGATTCCTATATTCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCAT
TTAATGCAGGGCTTGTTCAGCCGTGCACTTTAAGAGTAGCCTACCGCGCCAGTTTTGCCAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References