Detailed information of TA system TA233324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3887978..3888671
• Replicon: chromosome
• Accession: NZ_CP091427
• Organism: Escherichia coli strain 1000C-3

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: L3Z43_RS18595
• Protein ID: WP_000415584.1
• Coordinates: 3888375..3888671 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: L3Z43_RS18590
• Protein ID: WP_000650107.1
• Coordinates: 3887978..3888373 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3Z43_RS18580 (3883842)	3883842..3886100	-	2259	WP_032329482.1	DNA topoisomerase IV subunit A	-
L3Z43_RS18585 (3886238)	3886238..3887845	-	1608	WP_116290049.1	ABC transporter substrate-binding protein	-
L3Z43_RS18590 (3887978)	3887978..3888373	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
L3Z43_RS18595 (3888375)	3888375..3888671	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
L3Z43_RS18600 (3888876)	3888876..3889358	-	483	WP_000183505.1	GyrI-like domain-containing protein	-
L3Z43_RS18605 (3889411)	3889411..3889803	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
L3Z43_RS18610 (3889955)	3889955..3890614	+	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
L3Z43_RS18615 (3890611)	3890611..3891960	+	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
L3Z43_RS18620 (3892006)	3892006..3892338	-	333	WP_000917685.1	DUF2645 family protein	-
L3Z43_RS18625 (3892657)	3892657..3893238	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
L3Z43_RS18630 (3893269)	3893269..3893583	+	315	WP_000958598.1	putative quinol monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T233324 WP_000415584.1 NZ_CP091427:c3888671-3888375 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T233324 NZ_HG738868:1628899-1629213 [Serratia marcescens SMB2099]
ATGCAATTTACGGTGTATGCGAATAGGGGGAACAGCGCGGTTTATCCCCTGCTGCTTGATGTGACGAGCGATATCATTGG
GCAATTAAATCGTCGAGTTGTGATCCCTTTGTTGCCCGTTGAAAAATACCCGGGGAGTACGCGACCGGAACGCTTGATCC
CGTTGATCAGATTGATTGATGACAACGAGTACGCCGTGATGACATATGAAATGGCCAGCATCCCGGTTCGGGCTTTGGGG
GCCGAGTTTTGTGATGTTTCCCAGTATCGGACCAGGATAAAAGCCGCCATCGACTTTCTTCTTGACGGTATTTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT233324 WP_000650107.1 NZ_CP091427:c3888373-3887978 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT233324 NZ_HG738868:1628660-1628896 [Serratia marcescens SMB2099]
ATGCCAGTAAAACAACGAACGACGCAAAGTGTGACCATGACGGTGGAACGCAGTTTGTTGAGCCGTGCGCGTGAGGCGGG
GATTAATCTCAGCGCGACGCTAACTGCTGCTTTAGATGCTGAGTTACGTCAGCATGAGAGGATAAAATGGCAGGAAGAAA
ACCGAGAGGCGCTTGAAGCGCTTAACCGATTTAATGATGAGCATGGTTGCTTTAGCGACGAATACAGGACGTTTTAG

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65