Detailed information of TA system TA233307

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 843641..844335
• Replicon: chromosome
• Accession: NZ_CP091427
• Organism: Escherichia coli strain 1000C-3

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: L3Z43_RS04010
• Protein ID: WP_001263489.1
• Coordinates: 843937..844335 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: L3Z43_RS04005
• Protein ID: WP_000554758.1
• Coordinates: 843641..843934 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3Z43_RS03985 (839273)	839273..839770	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
L3Z43_RS03990 (839994)	839994..841706	-	1713	Protein_773	flagellar biosynthesis protein FlhA	-
L3Z43_RS03995 (841678)	841678..842463	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
L3Z43_RS04000 (842534)	842534..843589	+	1056	WP_001226164.1	DNA polymerase IV	-
L3Z43_RS04005 (843641)	843641..843934	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
L3Z43_RS04010 (843937)	843937..844335	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
L3Z43_RS04015 (844345)	844345..844797	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
L3Z43_RS04020 (845115)	845115..845321	+	207	Protein_779	RtcB family protein	-
L3Z43_RS04025 (845317)	845317..845838	+	522	Protein_780	peptide chain release factor H	-
L3Z43_RS04030 (845895)	845895..847352	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
L3Z43_RS04035 (847613)	847613..848071	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (848667)	848667..848747	+	81	NuclAT_11	-	-
- (848667)	848667..848747	+	81	NuclAT_11	-	-
- (848667)	848667..848747	+	81	NuclAT_11	-	-
- (848667)	848667..848747	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	837597..862268	24671

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T233307 WP_001263489.1 NZ_CP091427:843937-844335 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T233307 NZ_HG738867:c1978666-1978415 [Escherichia coli str. K-12 substr. MC4100]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT233307 WP_000554758.1 NZ_CP091427:843641-843934 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT233307 NZ_HG738867:c1978418-1978164 [Escherichia coli str. K-12 substr. MC4100]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3