Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 837597..838138 Replicon chromosome
Accession NZ_CP091427
Organism Escherichia coli strain 1000C-3

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag L3Z43_RS03970 Protein ID WP_000615983.1
Coordinates 837597..837875 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag L3Z43_RS03975 Protein ID WP_000729703.1
Coordinates 837878..838138 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3Z43_RS03955 (835179) 835179..835757 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
L3Z43_RS03960 (835963) 835963..836730 + 768 WP_000333380.1 class II glutamine amidotransferase -
L3Z43_RS03965 (836701) 836701..837441 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
L3Z43_RS03970 (837597) 837597..837875 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
L3Z43_RS03975 (837878) 837878..838138 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
L3Z43_RS03980 (838348) 838348..839097 + 750 WP_000056849.1 C40 family peptidase -
L3Z43_RS03985 (839273) 839273..839770 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
L3Z43_RS03990 (839994) 839994..841706 - 1713 Protein_773 flagellar biosynthesis protein FlhA -
L3Z43_RS03995 (841678) 841678..842463 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 837597..862268 24671


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T233306 WP_000615983.1 NZ_CP091427:c837875-837597 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T233306 NZ_HG738867:c1978418-1978164 [Escherichia coli str. K-12 substr. MC4100]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT233306 WP_000729703.1 NZ_CP091427:c838138-837878 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT233306 NZ_HG738867:c1978666-1978415 [Escherichia coli str. K-12 substr. MC4100]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References