Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 284411..284952 Replicon chromosome
Accession NC_011741
Organism Escherichia coli IAI1

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag ECIAI1_RS01300 Protein ID WP_000615976.1
Coordinates 284411..284689 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag ECIAI1_RS01305 Protein ID WP_000729704.1
Coordinates 284692..284952 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECIAI1_RS01285 281993..282571 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
ECIAI1_RS01290 282777..283544 + 768 WP_012599978.1 class II glutamine amidotransferase -
ECIAI1_RS01295 283515..284255 - 741 WP_001225679.1 murein L,D-transpeptidase -
ECIAI1_RS01300 284411..284689 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
ECIAI1_RS01305 284692..284952 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
ECIAI1_RS01310 285138..285911 + 774 WP_000543897.1 C40 family peptidase -
ECIAI1_RS01315 285968..286324 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
ECIAI1_RS01320 286317..286595 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
ECIAI1_RS01325 286700..288412 - 1713 Protein_248 flagellar biosynthesis protein FlhA -
ECIAI1_RS01330 288384..289169 + 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T23326 WP_000615976.1 NC_011741:c284689-284411 [Escherichia coli IAI1]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T23326 NC_011741:c284689-284411 [Escherichia coli IAI1]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT23326 WP_000729704.1 NC_011741:c284952-284692 [Escherichia coli IAI1]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT23326 NC_011741:c284952-284692 [Escherichia coli IAI1]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References