Detailed information of TA system TA023325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4471898..4472697
• Replicon: chromosome
• Accession: NC_011740
• Organism: Escherichia fergusonii ATCC 35469

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: B7LMV3
• Locus tag: EFER_RS21775
• Protein ID: WP_000347277.1
• Coordinates: 4471898..4472362 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: B7LMV4
• Locus tag: EFER_RS21780
• Protein ID: WP_015953962.1
• Coordinates: 4472362..4472697 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFER_RS21745	4466900..4467334	-	435	WP_000948832.1	PTS sugar transporter subunit IIA	-
EFER_RS21750	4467352..4468230	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
EFER_RS21755	4468220..4468999	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
EFER_RS21760	4469010..4469483	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
EFER_RS21765	4469506..4470786	-	1281	WP_000681964.1	tagatose-bisphosphate aldolase subunit KbaZ	-
EFER_RS21770	4471034..4471843	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
EFER_RS21775	4471898..4472362	-	465	WP_000347277.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
EFER_RS21780	4472362..4472697	-	336	WP_015953962.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
EFER_RS21785	4472846..4474417	-	1572	WP_000247251.1	galactarate dehydratase	-
EFER_RS21790	4474888..4475658	+	771	WP_001058567.1	2-dehydro-3-deoxyglucarate aldolase	-
EFER_RS21795	4475683..4476573	+	891	WP_000178101.1	2-hydroxy-3-oxopropionate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T23325 WP_000347277.1 NC_011740:c4472362-4471898 [Escherichia fergusonii ATCC 35469]
MDFPQRVNGWALYTHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Nucleotide

• Length: 465 bp

>T23325 NC_011740:c4472362-4471898 [Escherichia fergusonii ATCC 35469]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATACTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAGCCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12281.89 Da
• Isoelectric Point: 4.4128

>AT23325 WP_015953962.1 NC_011740:c4472697-4472362 [Escherichia fergusonii ATCC 35469]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDPTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT23325 NC_011740:c4472697-4472362 [Escherichia fergusonii ATCC 35469]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGATTGGGAGATGAACAGGAGGATCCTACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: B7LMV3

Antitoxin

• AlphaFold DB: B7LMV4