Detailed information of TA system TA233238

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 960139..960724
• Replicon: chromosome
• Accession: NZ_CP091361
• Organism: Acinetobacter baumannii strain AB177-VUB

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: L2Z13_RS04715
• Protein ID: WP_000897307.1
• Coordinates: 960139..960459 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L2Z13_RS04720
• Protein ID: WP_000369781.1
• Coordinates: 960452..960724 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2Z13_RS04680 (L2Z13_04680)	955894..956208	-	315	WP_004728120.1	BrnA antitoxin family protein	-
L2Z13_RS04685 (L2Z13_04685)	956195..956482	-	288	WP_000438825.1	BrnT family toxin	-
L2Z13_RS04690 (L2Z13_04690)	956751..957281	-	531	WP_000172759.1	hypothetical protein	-
L2Z13_RS04695 (L2Z13_04695)	957430..957732	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
L2Z13_RS04700 (L2Z13_04700)	957733..958014	+	282	WP_000985609.1	putative addiction module antidote protein	-
L2Z13_RS04705 (L2Z13_04705)	958094..958513	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
L2Z13_RS04710 (L2Z13_04710)	958676..960010	+	1335	WP_033917544.1	ISNCY family transposase	-
L2Z13_RS04715 (L2Z13_04715)	960139..960459	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L2Z13_RS04720 (L2Z13_04720)	960452..960724	+	273	WP_000369781.1	NadS family protein	Antitoxin
L2Z13_RS04725 (L2Z13_04725)	961217..962692	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
L2Z13_RS04730 (L2Z13_04730)	962748..963632	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sul2	-	947201..960010	12809
	-	inside	IScluster/Tn	sul2 / msr(E) / mph(E) / blaADC-25	-	950340..969147	18807

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T233238 WP_000897307.1 NZ_CP091361:960139-960459 [Acinetobacter baumannii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T233238 NZ_HF571988:885809-886234 [Yersinia enterocolitica (type O:5) str. YE53/03]
GTGGCCCAGTGGCGATGTGACCTGCGGATATCCTGGCACACTCAACTCTTCTCTCTATTAGCTCATGGCGTCCTGGTCAC
ACTGACGCTGGTTGCGCCTTGGCCTCAGGGCTATACCGCGCTGTGGTTGGTGTTACTGACGTTAGTGGTGTTTGAGTGTA
TCCGCAGCCAGAAAAATATCACGTCTTGCCAAGGCGAGATCAGGCTGAAACCCGGCAATATAGTGCTATGGAAACGACAT
GAGTGGGTTGTCATTAAGCAGCCGTGGATCACCCGTTATGGCGTGTTATTGAGTTTACAACAGACAAGCAACCGCTCAAC
CCGTAAGCGTTTATGGTTAGCGGCTGATAGCATGTCTGAAGAGGAATGGCGGCAATTGTGTCTCCTGTTGCGCCATTCAT
TTGGCTCCGATGAAGGTATTAATTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT233238 WP_000369781.1 NZ_CP091361:960452-960724 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT233238 NZ_HF571988:885562-885828 [Yersinia enterocolitica (type O:5) str. YE53/03]
ATGGAAATCGATAATAAAGCCCGTATCCACTGGGCGTGCCGTCGGGGTATGCGTGAACTGGATATCTCTATCATGCCGTT
TTTTGAACATGAATATGATGGCTTGAGCGATAACGAGAAACAGGTTTTTATCCGTTTGCTTGAGTGTGATGATCCAGATT
TATTTAACTGGTTGATGAATCACGGGGAACCGCAGGATAGTGAGCTGCGTCAAATGGTCAAACTGATTCAAACACGAAAT
AAAGCCCGTGGCCCAGTGGCGATGTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3