Detailed information of TA system TA233224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 3011310..3011895
• Replicon: chromosome
• Accession: NZ_CP091356
• Organism: Acinetobacter baumannii strain AB186-VUB

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: L2Z19_RS14320
• Protein ID: WP_000897307.1
• Coordinates: 3011575..3011895 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L2Z19_RS14315
• Protein ID: WP_000369781.1
• Coordinates: 3011310..3011582 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2Z19_RS14300 (L2Z19_14285)	3007220..3008310	+	1091	WP_085940413.1	IS4-like element ISAba1 family transposase	-
L2Z19_RS14305 (L2Z19_14290)	3008402..3009286	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
L2Z19_RS14310 (L2Z19_14295)	3009342..3010817	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
L2Z19_RS14315 (L2Z19_14300)	3011310..3011582	-	273	WP_000369781.1	NadS family protein	Antitoxin
L2Z19_RS14320 (L2Z19_14305)	3011575..3011895	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L2Z19_RS14325 (L2Z19_14310)	3012024..3013358	-	1335	WP_033917544.1	ISNCY family transposase	-
L2Z19_RS14330 (L2Z19_14315)	3013521..3013940	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
L2Z19_RS14335 (L2Z19_14320)	3014020..3014301	-	282	WP_000985609.1	putative addiction module antidote protein	-
L2Z19_RS14340 (L2Z19_14325)	3014302..3014604	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
L2Z19_RS14345 (L2Z19_14330)	3014753..3015283	+	531	WP_000172759.1	hypothetical protein	-
L2Z19_RS14350 (L2Z19_14335)	3015552..3015839	+	288	WP_000438825.1	BrnT family toxin	-
L2Z19_RS14355 (L2Z19_14340)	3015826..3016140	+	315	WP_004728120.1	BrnA antitoxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	sul2	-	3012024..3020962	8938
	-	inside	IScluster/Tn	blaADC-25 / mph(E) / msr(E) / sul2	-	3002887..3021694	18807

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T233224 WP_000897307.1 NZ_CP091356:c3011895-3011575 [Acinetobacter baumannii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T233224 NZ_FO834906:358530-358877 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT233224 WP_000369781.1 NZ_CP091356:c3011582-3011310 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT233224 NZ_FO834906:358877-359176 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGCCCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3