Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 4468755..4469341 Replicon chromosome
Accession NZ_CP091330
Organism Klebsiella pneumoniae strain CRK3022

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag L3Y35_RS21320 Protein ID WP_002920800.1
Coordinates 4468973..4469341 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag L3Y35_RS21315 Protein ID WP_004174006.1
Coordinates 4468755..4468976 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3Y35_RS21295 (4464912) 4464912..4465838 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
L3Y35_RS21300 (4465835) 4465835..4467112 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
L3Y35_RS21305 (4467109) 4467109..4467876 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
L3Y35_RS21310 (4467878) 4467878..4468591 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
L3Y35_RS21315 (4468755) 4468755..4468976 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
L3Y35_RS21320 (4468973) 4468973..4469341 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
L3Y35_RS21325 (4469614) 4469614..4470930 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
L3Y35_RS21330 (4471037) 4471037..4471924 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
L3Y35_RS21335 (4471921) 4471921..4472766 + 846 WP_004181473.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
L3Y35_RS21340 (4472768) 4472768..4473838 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4465835..4474575 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T233181 WP_002920800.1 NZ_CP091330:4468973-4469341 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T233181 NZ_FO082820:475189-475512 [Pseudorhizobium banfieldiae]
ATGAGGCGCGGCGATCTTGTCACGATCGCTCTTCCAGGTGATTTTTCGAAGCCTCGACCAGCGCTGATCATCCAATCAGA
TCTTTTCGCCGAAACAGGAACGGTTACCGTGCTGCTGGTTTCGGCGACGATCGTCGAGGCGCCCTTGATCCGAGTGACTG
TGCATCCTTCGGCGGCGAACCGGCTTCGCAAGATTTCTCAGGTGATGGTCGACAAGGCAATGTCCGTGAAGCGCGACAAG
CTTGGTCCGACGTTTGGACGCCTTGAGGATGAGAGTATGCTTGCAGTCAACCGTTCCCTGGCTGTCTTTCTCAATATTCT
CTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT233181 WP_004174006.1 NZ_CP091330:4468755-4468976 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT233181 NZ_FO082820:474968-475192 [Pseudorhizobium banfieldiae]
ATGGCGATCCCGGTCAACAAGCGTGTGGAAAAGCGGCGCGAGGCACTGAGACTGGCGGGGCTGAGGCCTGTTCAGATCTG
GGTGCCGGACACTCGGCAAGAAGGGTTCGTTGCGGAGTGCCGCCGTCAGGCCTCGCTGGTTGCAGCCGCGGATGCCATAG
ATGCGGATCTCGACGCCTTCCTGCAAGCCGCGCTGGGCCAGTTGGATAGAGACGGCGAGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References