Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2706806..2707403 Replicon chromosome
Accession NZ_CP091330
Organism Klebsiella pneumoniae strain CRK3022

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag L3Y35_RS13100 Protein ID WP_004142563.1
Coordinates 2707086..2707403 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag L3Y35_RS13095 Protein ID WP_004142561.1
Coordinates 2706806..2707093 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3Y35_RS13065 (2702886) 2702886..2703134 + 249 WP_020325243.1 DUF1158 domain-containing protein -
L3Y35_RS13070 (2703152) 2703152..2703493 - 342 WP_025403994.1 RamA family antibiotic efflux transcriptional regulator -
L3Y35_RS13075 (2703524) 2703524..2704639 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
L3Y35_RS13080 (2704819) 2704819..2705400 + 582 WP_215402451.1 TetR/AcrR family transcriptional regulator -
L3Y35_RS13085 (2705400) 2705400..2705768 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
L3Y35_RS13090 (2705888) 2705888..2706541 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
L3Y35_RS13095 (2706806) 2706806..2707093 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
L3Y35_RS13100 (2707086) 2707086..2707403 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L3Y35_RS13105 (2707588) 2707588..2708631 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
L3Y35_RS13110 (2709301) 2709301..2710167 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
L3Y35_RS13115 (2710276) 2710276..2711703 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T233175 WP_004142563.1 NZ_CP091330:c2707403-2707086 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T233175 NZ_CP128550:5399605-5400072 [Pseudomonas alloputida]
ATGAGTGATGCGAGCAGGAAGCCTCTGGTTATTCATGGATGGACTGTAATTGCCCACCCGCTGTTCCTGGCCAAACTGGA
CGCGCTAAGCGCTCAAGTCGAGGCTCAACGGGACAAAGACCCAACGGGTTATACGAAAAGAAATACCTTTAAAAGGCTGG
CCGCCATCCGGCGGTTGGCGTTCGATGTAATCCCTCAAGACCCGACCAAACCCGAATACCGGCAAGGGGCAACACTTGGC
GGTGACCACAAGCACTGGTTCAGAGCAAAGTTCTTCCAGCAGTACCGGTTGTTCTTCCGCTACCACACCCCCAGCAGGAT
CATCGTGCTGGCCTGGATCAATGACGAGTCGAGCAAGCGGGCCTATGAAAGTAGCGACGATGCTTACAAGGTCTTTCAAA
AGATGTTGCATAGCGGCAACCCACCGGACGATTGGGACCAGTTGCTGCAAGAGGCTGCCGCAGATTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT233175 WP_004142561.1 NZ_CP091330:c2707093-2706806 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT233175 NZ_CP128550:5399282-5399608 [Pseudomonas alloputida]
ATGGCTGCCACCTTCGACGTCGAGTCCACGCTGACCGATCGCTATCAAACCACTGTTCCCGAAACGGTGCGGCGCGCCTT
GGGCTTGAAAAAACGTGACAAGATCCATTACTCGATTCGCCCCGACGGTGGGGTGATACTCACCCGCGCAGAGGCAACCG
AGGACGATCCGGTACTGGGCAAATTTCTGGATTTCCTGGCACACGATATCGCTACCAACCCTCAACGGCTGCAGGTTGTC
GATGCTGGCCTCATCGCGCGCCTCGACAAGCTTGTCGGCGACGCGGAGGTCGACCTGGATCAACCTCTGTCGGCGGACGA
TGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References